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Intact Genomics has developed and manufactured a variety of kits and enzymes useful for various cloning needs. These include our propriatary Quick10™ and igFusion™ Cloning Kits as well as various other products useful for optimizing your molecular cloning workflow.
| Product Links | Size Available | Application & Benefits |
|---|---|---|
| Tth DNA Ligase | 1,000 & 10,000 Units | Ideal for high-temperature, high-precision ligation assays, including SNP genotyping, mutation detection, LCR, NGS library preparation, and synthetic biology applications. |
| Taq DNA Ligase | 10,000 Units | Ideal for routine ligation-based assays in cost-sensitive workflows, including SNP genotyping, mutation detection, and LCR. |
| T4 DNA Ligase | 100,000 & 400,000 Units | Ideal for general DNA ligation, including cloning, vector construction, sticky-end and blunt-end ligation, and NGS library preparation. |
| T4 Polynucleotide Kinase (PNK) | 2,500 Units | Used for DNA/RNA end modification, including 5′-end phosphorylation, dephosphorylation (3′-phosphatase activity), labeling, and NGS library preparation. |
| T4 DNA Polymerase | 500 Units | Used for DNA end repair, blunting of overhangs, second-strand synthesis, and NGS library preparation. |
| Quick10™ Cloning Kit | 6 Reactions | Enables assembly-to-plate cloning in as little as 10 minutes. |
| ig-Fusion™ Cloning Kit | 10, 50 & 100 Reactions | Clone any PCR product(s) into any linearized expression vector at any site using fusion cloning technology. |
| ig-Fusion™ Cloning Enzyme Premix | 10, 50 & 100 Reactions | Standalone ig-Fusion™ enzyme premix for custom cloning workflows. |
| T5 Exonuclease | 5,000 Units | Commonly used for DNA assembly, seamless cloning, homologous recombination, and generation of single-stranded overhangs. |
| Exonuclease III (Exo III) | 10,000 Units, 25,000 Units | Used for controlled 3′→5′ digestion of double-stranded DNA in deletion mapping, DNA end polishing, and site-directed mutagenesis. |
Utilizing Intact Genomics Quick10™ Cloning kit, DNA/PCR product(s) can be assembled/transformed in 10 minutes vs. other seamless/fusion cloning, conventional T/A, or restriction ligation cloning and transformation methods that can take up to 2 hours or more.
Benefits Include
Combination of high fidelity PCR, assembly, and DirectPlate® transformation cloning technologies.
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Combination of homologous assembly and ccdB selection(vs. T/A or restriction ligation methods) display <1% false-positive clones or nearly 100% cloning accuracy.
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Assembled/transformed in 10 minutes. Less time and less effort spent on cloning, transformation, and positive clone screening/identification.
Applications
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Streamlined cloning of one or two (<6 kb total) DNA fragments
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Single PCR product cloning
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Site directed mutagenesis
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High throughput cloning
Product Includes
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5x Quick10-Assemble™ Premix
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IG® AmpR/KanR-pCR vector
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Dilution buffer
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Directplate® XL 10B competent cells (6×50 µl)
igFusion™ Cloning Kit
Intact Genomics propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. he kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 min) with more than 95% cloning efficiency. 5x ig-Fusion™ enzyme premix also sold separately.
Benefits Include
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Clone any insert at any site within any vector
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Restriction enzyme and phosphatase free system
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Joining multiple large fragments at once
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Precise insertion at a desired orientation
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Rapid and high efficiency with > 95% positive clones