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Reverse Transcription Enzymes and Kits - Intact Genomics
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Intact Genomics has developed and manufactured a variety of kits and enzymes useful for various Reverse transcription needs. All kits and products contain Intact Genomics proprietary igScript™ Reverse Transcriptase.
Product Links Size Available Application & Benefits Glycerol-free RNase Inhibitor, Murine 5,000 & 20,000 Units A powerful tool to inhibit and control RNase contaminants in enzymatic manipulations of RNA RNase Inhibitor, Murine 5,000 & 20,000 Units A powerful tool to inhibit and control RNase contaminants in enzymatic manipulations of RNA igScript™ Glycerol-free Reverse Transcriptase 10,000 & 50,000 Units A recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates igScript™ Reverse Transcriptase 50,000 Units A recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates igScript™ First Strand cDNA Synthesis Kit 25 & 100 Reactions First strand cDNA synthesis for PCR or RT-PCR, active at 55°C igScript™ One Step RT-PCR Kit 100 & 500 Reactions igScript™ One Step RT-PCR kit combines two powerful mixtures: i) igScript™ Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. igScript™ One Step RT-qPCR Kit 100 & 500 Reactions Combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix igScript™ Probe-Based One Step RT-qPCR Kit 200, 500, 1,000, & 5,000 Reactions Single tube option for robust Probe-Based RT-qPCR igScript™ Probe-Based RT-qPCR Kit 50, 100, & 500 Reactions Combines two powerful mixtures: i). igScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix with standard buffer providing improved PCR efficiency T7 RNA Polymerase 5,000U & 25,000U T7 RNA Polymerase is useful for sgRNA and mRNA synthesis, RNA synthesis from DNA templates and applications in mRNA therapeutics
igScript™ Reverse Transcriptase Related Products
igScript™ Reverse Transcriptase (igScript™ RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. igScript™ RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.
Key Benefits
Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity.
Active at temperatures up to 55°C.
Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA.
Available in many varieties including in our cDNA Synthesis kit (which includes 5x igScript™ master mix, Oligo and Hexamer Primers, and nuclease free water)
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