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Taq DNA Polymerase 2x Premix - Intact Genomics
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Taq DNA Polymerase 2x Premix
$ 144.00– $ 273.00Price range: $144.00 through $273.00
Description
IG™ Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.
IG™ Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2 , and stabilizers. It has been optimized for routine PCR applications.
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).
Product Source Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Applications
PCR
Primer extension
Colony PCR
Product Includes
Taq DNA Polymerase 2x Premix with Dye
5x Magic Enhancer
Storage Temperature –20 °C
1x Premix Composition
10 mM Tris-HCl pH 9.0 , 50 mM KCl, 1.5 mM MgCl2 , 0.2 mM dNTP, 5% Glycerol, 0.08% Igepal CA 630, 0.05% TWEEN-20, 25 Units/ml Taq DNA Polymerase
Unit Definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 ºC.
Product Use Limitation and Disclaimers
This product is for research purposes only. It is not intended for therapeutic or diagnostic purposes in humans or
Technical Support
Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.
3248 3249 3250
Thaw primer solutions, 5x Magic Enhancer (if required) and mix thoroughly before use.
Prepare a reaction mix according to the following table: The reaction mix typically contains all the components needed for PCR except the template DNA.
Mix the reaction mixture thoroughly. Add template DNA to the individual PCR tubes containing the reaction mixture.
Program the thermal cycler according to the manufacturer’s instructions.
A typical PCR cycling program is outlined in the following table.
Place the PCR tubes in the thermal cycler and start the cycling program.
Reference
EChien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. 127, 1550-1557.
Lawyer, F.C. et al. (1993). PCR Methods and Appl. 2, 275-287.
Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res. 18, 7317-7322.
Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
Manual - Taq DNA Polymerase 2x Premix with Dye
MSDS - ig Taq DNA Polymerase 2x Premix with Dye
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