Intact Genomics T4 DNA Polymerase has both a DNA-dependent DNA polymerase activity and a potent 3´→5´ exonuclease activity.  

The purity of this enzyme is > 95% homogeneity as determined by SDS-PAGE using Coomassie® Blue staining (see figure below).


 Product SourceE.coli cells with a cloned gene of bacteriophage T4 DNA Polymerase.


  • 3´-overhang removal to form blunt ends (1, 2).
  • 5´-overhang fill-in to form blunt ends (1, 2).
  • Probe labeling using replacement synthesis (2).
  • DNA library preparation for Next-generation sequencing.
  • Ligation-independent cloning of PCR products.
  • Second strand synthesis in site-directed mutagenesis (3).

Product Includes

  1. T4 DNA Polymerase
  2. 10x T4 DNA Polymerase Buffer

Storage Temperature
–20 °C

Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA,
50% Glycerol, pH 7.5 @ 25 ºC

10X T4 DNA Polymerase Reaction Buffer

500 mM Tris-HCl, 100 mM MgCl2, 50 mM dithiothreitol, pH 7.5 @ 25°C.

Unit Definition

One unit of T4 DNA Polymerase converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard assay conditions.


Inactivated by heating at 70°C for 15 min.

Quality Control Assays

T4 DNA Polymerase is free from detectable endonuclease and RNase activities.3221 3222