Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™  DH5-Alpha chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™  DH5-Alpha chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.

DirectPlate Protocol Comparison 2022

Please find a video below displaying the convenience and capability of this product.


Competent cell type:  Chemically competent
Derivative of:  DH5-Alpha
Species:  E. coli
Format:  Tubes
Transformation efficiency: ≥1 x 108 – 109 cfu/µg pUC19 DNA
Blue/white screening:  Yes
Shipping condition:  Dry ice

Reagents Needed for One Reaction

DirectPlate™ DH5-Alpha competent cell:  50 µl
DNA (or pUC19 Control, 10 pg/µl):  1 µl

Storage of the DH5-Alpha Competent Cell

DirectPlate™  DH5-Alpha competent cells:   -80 ºC
pUC19 control DNA:  -20 ºC


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Quality Control

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×108 – 109 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

Follow these guidelines when using DirectPlate™  DH5-Alpha chemically competent E. coli.

  • Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
  • Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by gently pipetting up and down a few times. 1013-13 1013-36

Comparison Testing

Intact Genomics has performed extensive comparison testing to prove our DirectPlate™  DH5-Alpha chemically competent cells have the highest transformation efficiency on the market.

Direct Plate vs ZYMO TG-1 Phage Display Competent Cell ComparisonAdditional experimental details are available upon request.
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