Intact Genomics offers a variety of RPA optimization products.  RPA is an excellent candidate for developing rapid point-of-care molecular testing tools as well as agricultural applications, clonal amplification in next-generation sequencing workflows, and more.

Product Links	Pakage Size	Features
T4 UvsX DNA Recombinase	100 μg, 500 μg, & 1000 µg	Important for the error-free repair of DNA double-strand breaks and for replication fork restart
T4 UvsY Protein	100 μg, 500 μg, & 1000 µg	T4 UvsY is the phage T4 recombination mediator protein. It stimulate sthe filament nucleation event
T4 gp32 Protein	500 μg	Single-stranded DNA binding protein required for T4 DNA replication, recombination, and repair
Bsu DNA Polymerase Large Fragment	1,000 units	Polymerase useful for extending primers in RPA
Sau DNA Polymerase Large Fragment	1,000 units	Polymerase useful for extending primers in RPA
Glycerol Free T4 UvsX DNA Recombinase	100 μg, 500 μg, & 1000 µg	Important for the error-free repair of DNA double-strand breaks and for replication fork restart
Glycerol Free T4 UvsY Protein	100 μg, 500 μg, & 1000 µg	T4 UvsY is the phage T4 recombination mediator protein. It stimulate sthe filament nucleation event
Glycerol Free T4 gp32 Protein	500 μg	Single-stranded DNA binding protein required for T4 DNA replication, recombination, and repair
Glycerol Free Bsu DNA Polymerase Large Fragment	1,000 units	Polymerase useful for extending primers in RPA

Recombinase polymerase amplification (RPA) amplifies DNA at a constant temperature (37-42 °C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX is used in combination with its accessory protein, UvsY. The recombinase interacts with the primers to form nucleoprotein filament. This complex is able to bind with homologous double-stranded DNA through a strand exchange. After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu/Sau polymerase extends the primers, creating a new complete copy of the template, and amplification can continue like Polymerase Chain Reaction (PCR).

Intact Genomics offers a variety of RPA optimization enzymes. We are continuously discovering and developing novel classes of RPA enzymes in order to speed up the point-of-care (POC) diagnostic process.