Agrobacterium Competent Cells


Agrobacterium tumefaciens, Agrobacterium rhizogenes, and Methionine- and Thymidine-Auxotrophic Agrobacterium Strains

High Efficiency Chemically & Electro Agrobacterium Competent Cells

Intact Genomics (IG) is the largest and earliest provider of agrobacterium competent cells in the market. We provide the highest quality agrobacterium competent cells to customers and distributors worldwide. We also developed the first high-efficiency chemically agrobacterium competent cells in 2020. Using propriety methods, Intact Genomics has developed and manufactured a variety of Agrobacterium competent cells to support the development of plant transformation and agricultural research.

Scroll down to find our collection of Agrobacterium tumefaciens, agrobatecium rhizogens and Methionine and Thymidine Auxotrophic Agrobacteria competent cells.

Tomatoes, corn plant, and corn cobs

Agrobacterium tumefaciens Competent Cells

Agrobacterium tumefaciens was first isolated from the gall tissue and identified as the cause of crown gall disease (Smith and Townsend, 1907). Over 100 years study of A. tumefaciens has not only revolutionized plant molecular genetics and breeding, but also given birth to a whole new biotech industry dedicated to the genetic transformation of plants and the production of  recombinant proteins by plant tissues. In nature, species or strains of  agrobacteria infect more than 600 members of most of the plant families. IG Agrobacterium tumefaciens strains include EHA105, LBA4404, GV3101, AGL-1 and more. All are useful for plant transgenic operations in a variety of species.

Product Links Efficiency Application & Benefits
GV3101 Agrobacterium Chemically Competent Cells ≥1.0 × 10⁵ Transgenic operations of Arabidopsis thaliana, tobacco, corn, potato, and other plants.
GV3101 Agrobacterium ElectroCompetent Cells ≥1.0 × 10⁷
AGL1 Agrobacterium Chemically Competent Cells ≥1.0 × 10⁵ Transgenic operations of Arabidopsis thaliana, maize and other monocots.
AGL1 Agrobacterium ElectroCompetent Cells ≥1.0 × 10⁷
LBA4404 Agrobacterium Chemically Competent Cells ≥1.0 × 10⁵ Transgenic operations of tomatoes, tobacco and other plants.
LBA4404 Agrobacterium ElectroCompetent Cells ≥1.0 × 10⁷
EHA105 Agrobacterium Chemically Competent Cells ≥1.0 × 10⁵ Transgenic operations of rice, tobacco and other plants.
EHA105 Agrobacterium ElectroCompetent Cells ≥1.0 × 10⁷
C58C1 Agrobacterium Chemically Competent Cells ≥1.0 × 10⁵ Transgenic operations involving potatoes, tobacco and other plants.
C58C1 Agrobacterium ElectroCompetent Cells ≥1.0 × 10⁷
Agrobacterium Chemically Competent Cells Combo Pack Various For applications requiring multiple Agrobacterium competent cell strains.
Agrobacterium ElectroCompetent Cells Combo Pack Various
GV3101 (pSoup) Agrobacterium ElectroCompetent Cells ≥1.0 × 10⁷ Transgenic operations of Arabidopsis thaliana, tobacco, corn, potato, and other plants.
GV3101 (pSoup-p19) Agrobacterium ElectroCompetent Cells ≥1.0 × 10⁷ Transgenic operations of Arabidopsis thaliana, tobacco, corn, potato, and other plants.
AGL1 (pSoup) Agrobacterium ElectroCompetent Cells ≥1.0 × 10⁷ Transgenic operations of Arabidopsis thaliana, maize and other monocots.
EHA105 (pSoup) Agrobacterium ElectroCompetent Cells ≥1.0 × 10⁷ Transgenic operations of rice, tobacco and other plants.

Agrobacterium rhizogenes Competent Cells

Agrobacterium rhizogenes (or Rhizobium rhizogenes) is a Gram-negative soil bacterium that produces hairy root disease in dicotyledonous plants. Hairy root culture systems are considered as green factories for mass production of valuable molecules, because of their several attractive features, including high genetic and biochemical stability, and relatively fast growth rates in hormone-free media. Examples of pharmaceutically relevant molecules produced by transformed root culture include anticancer (e.g., paclitaxel and camptothecin), antimalarial (e.g., artemisinin; its discovery was awarded Nobel Prize in Physiology and Medicine in 2015), and anti-inflammatory (verbascoside) compounds, among others. Besides as green factories for high-value molecules biosynthesis A. rhizogenes–mediated transformations are utilized to elucidate biosynthetic pathways and physiological processes, generate recombinant therapeutic proteins, assist molecular breeding, and enhance phytoremediation efforts. IG Agrobacterium rhizogenes strains include AR.A4, K599, MSU440 and more. All are useful for plant transgenic operations in a variety of species.

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Product Links Efficiency Application & Benefits
Ar.A4 Agrobacterium Chemically Competent Cells ≥1.0 × 105 Transgenic operations of corn, tobacco, carrot and other plants.
Ar.A4 Agrobacterium ElectroCompetent Cells
ATCC15834 Agrobacterium Chemically Competent Cells Transgenic operations of legumes, tobacco, variety of grasses and other plants.
ATCC15834 Agrobacterium ElectroCompetent Cells
K599 Agrobacterium Chemically Competent Cells Transgenic operations of corn, soybean (wild soybean), cotton, peanut, dandelion,
cowpea and other plants.
K599 Agrobacterium ElectroCompetent Cells
MSU440 Agrobacterium Chemically Competent Cells Useful for transgenic operations of corn, tobacco, wormwood, tea tree and other plants.
MSU440 Agrobacterium ElectroCompetent Cells

Methionine and Thymidine Auxotrophic Agrobacteria Competent Cells

We also provide a variety of highly unique strains. This includes Methionine and Thymidine Auxotrophic Agrobacteria competent cells. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain. Auxotrophic Agrobacteria help to solve this problem. Methionine or Thymidine Auxotrophic Agrobacterium strains include modifications so that they will not grow unless methionine or thymidine is added to Minimal medium. Using the selections of minimal media without Methionine or Thymidine in combination with selective antibiotics, it completely prevents the bacteria from overgrowing plant tissues during plant transformation.

Intact Genomics’ Methionine Agrobacterium strains were originally cloned and provided by Dr. Wayne Parrott’s lab under license from University of Georgia. Thymidine Agrobacterium strains were originally cloned and provided by Dr. Kan Wang’s lab under license from Iowa State University. Knocking out genes to cause auxotrophy does not affect transformation capacity.

Benefits Include

  • Simple green science logo design

    Enables development of more efficient transformation systems.

  • Simple green science logo design

    Reduced bacterial overgrowth during co-cultivation.

  • Simple green science logo design

    Decreased need for antibiotics.

Product Links Efficiency Application & Benefits
Methionine Auxotrophic LBA4404 Agrobacterium Chemically Competent Cells ≥1.0 x 10^5 Transgenic operations of tomatoes, tobacco and other plants
Methionine Auxotrophic LBA4404 Agrobacterium ElectroCompetent Cells ≥1.0 x 10^7
Methionine Auxotrophic EHA105 Agrobacterium Chemically Competent Cells ≥1.0 x 10^5 Transgenic operations of rice, tobacco and other plants
Methionine Auxotrophic EHA105 Agrobacterium Electrocompetent Cells ≥1.0 x 10^7
Thymidine Auxotrophic EHA105 Agrobacterium Electrocompetent Cells ≥1.0 x 10^3 For electroporation transformation to rice, tobacco and other plants.
Thymidine Auxotrophic EHA101 Agrobacterium Electrocompetent Cells ≥1.0 x 10^3
Thymidine Auxotrophic EHA105D Agrobacterium Electrocompetent Cells ≥1.0 x 10^3
Thymidine Auxotrophic EHA105 Agrobacterium Chemically Competent Cells ≥1.0 x 10^3 For chemically transformation to rice, tobacco and other plants
Thymidine Auxotrophic EHA101 Agrobacterium Chemically Competent Cells ≥1.0 x 10^3
Thymidine Auxotrophic EHA105D Agrobacterium Chemically Competent Cells ≥1.0 x 10^3