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Struggling to express toxic or difficult proteins? Intact Genomics (IG®) C41(DE3) Chemically Competent Cells are engineered to improve tolerance to toxic protein expression while maintaining reliable T7-driven performance. Derived from BL21(DE3), C41(DE3) strain reduces cell death associated with membrane proteins, nucleases, and other challenging targets. When standard BL21(DE3) fails, C41(DE3) helps move your project forward. Custom formats (8-well strip, 96 or 384 well plate), custom package sizes and bulk pricing are available upon request. -
Built for Tough Protein Expression.
Designed for IPTG-inducible T7 systems, this BL21(DE3) derivative improves tolerance to toxic proteins while the pLysS plasmid suppresses basal expression for greater stability. Validated in 400+ publications, IG® C41pLysS(DE3) cells deliver dependable results for challenging protein expression. Custom formats (8-well strip, 96 or 384 well plate), package sizes and bulk pricing aare available upon request. -
Reliable Expression for Toxic Proteins.
IG® C43pLysS(DE3) Chemically Competent Cells are designed for high-efficiency transformation and stable expression of toxic or difficult proteins in IPTG-inducible T7 systems. Derived from BL21(DE3), this strain improves tolerance to toxic proteins, while the pLysS plasmid suppresses basal expression to stabilize challenging constructs.
Custom formats (8-well strip, 96 or 384 well plate), custom package sizes and bulk pricing are available upon request. -
IG® Autoinduction DE3 Chemically Competent Cells are suitable for transformation and routine recombinant protein expressed from a vector under an IPTG-inducible T7 promoter. With simple and less hands-on autoinduction, you can either enjoy more free-time or get more work done. Moreover, the yields of both expressed cells and protein are likely 5-10 fold or more for a broad range of protein targets at either small or large scale of protein production. -
Intact Genomics (IG®) Autoinduction DE3 ElectroCompetent cells are suitable for transformation and routine recombinant protein expressed from a vector under an IPTG-inducible T7 promoter. With simple and less hands-on autoinduction, you can either enjoy more free-time or get more work done. Moreover, the yields of both expressed cells and protein are likely 5-10 fold or more for a broad range of protein targets at either small or large scale of protein production. -
Intact Genomics (IG®) Autoinduction Medium promotes high-yield growth of E. coli and high-level expression of T7 promoter-regulated recombinant proteins without time-consuming steps such as monitoring optical density (OD) or adding induction components like IPTG. Unlike traditional LB-IPTG induction systems, IG® Autoinduction Medium allows regulated protein expression in any expression system that is solely inducible by IPTG from E. coli strains containing a fully functional lac operon. -
i7® High-Fidelity DNA Polymerase 2x master mix is a ready to use premix which contains high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® High-Fidelity DNA Polymerase 2x master mix has the high-fidelity, sensitivity and processivity with an error rate ~2.8x102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes.