Intact Genomics has developed and manufactured a variety of kits and enzymes useful for various Reverse transcription needs. All kits and products contain Intact Genomics proprietary igScript™ Reverse Transcriptase.
| Product Links | Size Available | Application & Benefits |
| Glycerol-free RNase Inhibitor, Murine | 5,000 & 20,000 Units | A powerful tool to inhibit and control RNase contaminants in enzymatic manipulations of RNA |
| RNase Inhibitor, Murine | 5,000 & 20,000 Units | A powerful tool to inhibit and control RNase contaminants in enzymatic manipulations of RNA |
| igScript™ Glycerol-free Reverse Transcriptase | 10,000 & 50,000 Units | A recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates |
| igScript™ Reverse Transcriptase | 50,000 Units | A recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates |
| igScript™ First Strand cDNA Synthesis Kit | 25 & 100 Reactions | First strand cDNA synthesis for PCR or RT-PCR, active at 55°C |
| igScript™ One Step RT-PCR Kit | 100 & 500 Reactions | igScript™ One Step RT-PCR kit combines two powerful mixtures: i) igScript™ Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. |
| igScript™ One Step RT-qPCR Kit | 100 & 500 Reactions | Combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix |
| igScript™ Probe-Based One Step RT-qPCR Kit | 200, 500, 1,000, & 5,000 Reactions | Single tube option for robust Probe-Based RT-qPCR |
| igScript™ Probe-Based RT-qPCR Kit | 50, 100, & 500 Reactions | Combines two powerful mixtures: i). IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix with standard buffer providing improved PCR efficiency |
| One Step RT-qPCR kit for SARSCoV-2 (COVID-19) detections | 100 & 500 Reactions | For in vitro detection of SARS-CoV-2 using Real-Time quantitative PCR (RT-qPCR), for RUO. |
igScript™ Reverse Transcriptase Related Products
igScript™ Reverse Transcriptase (igScript™ RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. igScript™ RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.
Key Benefits
- Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity.
- Active at temperatures up to 55°C.
- Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA.
- Available in many varieties including in our cDNA Synthesis kit (which includes 5x igScript™ master mix, Oligo and Hexamer Primers, and nuclease free water)