Description

Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.

The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).
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Product Source

E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Taq Polymerase Comparison Data

tt2 copy

 

 

 

 

 

Applications

  • Routing PCR cloning
  • Primer extension
  • Colony PCR
  • Elongation efficiency 1.0-1.2 kb/min.
  • Formulated for amplifying long target DNA.
  • Efficient for amplifying high GC content DNA with Intact Genomics magic enhancer

Product Includes

  1. Taq DNA Polymerase
  2. 10x PCR Buffer with Mg2+
  3. 5x Magic Enhancer
  4. 10 mM dNTP (Cat. # 3241d, 3241d-04 only)

Storage Temperature
–20 °C

Storage Buffer

50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 ºC

10x PCR Buffer with Mg2+

100 mM Tris-HCl pH 8.0, 15 mM MgCl2, 100 mM KCl, 80 mM (NH4)2SO4, 0.5% Igepal CA 630

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 ºC.

References:

  1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. 127, 1550-1557.
  2. Lawyer, F.C. et al. (1993). PCR Methods and Appl. 2, 275-287.
  3. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res. 18, 7317-7322.
  4. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.