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ig SYBR Green qPCR 2X Master Mix

Name: ig SYBR Green qPCR 2X Master Mix
SKU: 1750
Availability: InStock

$115.00 – $830.00

Categories: Enzymes, RT-PCR, RT-qPCR & qPCR.

Description

Intact Genomics SYBR® Green qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR Green qPCR 2x master mix with integrated chemically modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye*, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high-quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

Applications

Gene expression data validation.
Absolute quantification
Mutation detection
Pathogen detection
viral detection
Genetically modified organisms (GMO) characterization
Genetic profiling

Benefits

Enhanced efficiency, specificity, and sensitivity
Compatible with all real-time PCR instruments
Superior gene expression results under various
cycling conditions
Robust and active for cDNA synthesis at temperatures up to 55 °C.

Product Includes

ig™ SYBR Green qPCR 2x Master Mix

Storage Temperature

–20 °C

Note

*The use of ROX dye is necessary for all Applied Biosystems instruments and is optional for the Stratagene Mx3000P™, Mx3005P™, and Mx4000™ cyclers. Bio-Rad, Qiagen, Eppendorf, Illumina and Roche instruments do not require ROX dye. The concentration of ROX Dye is (5 nM) for this product.

Technical Support & Customer Services
Intact Genomics (IG®) is dedicated to customer satisfaction regarding the use of our products for your research
needs. Each new lot of our products is thoroughly tested to ensure it meets high quality standards and provides
excellent results. We appreciate your business and your feedback regarding the performance of our products in
your applications.

Additional information

reaction	200 reactions, 500 reactions, 2000 reactions

Please follow this protocol closely including the initial denaturation time and temperature.

Starting quantity of DNA can be 10pg-10ng. As little as 1pg may be acceptable with appropriate controls in place.

1. Place kit components, primers and RNA samples on ice.
2. Mix and then centrifuge briefly to collect contents at the bottom of the tube.
3. Prepare a master mix for each reaction and control plus 10% extra to allow for pipetting error according to the following table:

4. Mix the reaction mixture thoroughly.
5. Program the thermal cycler according to the manu-facturer’s instructions.
6. A typical PCR cycling program is outlined in the following table.

7. Place the PCR tubes in the thermal cycler and start the cycling program.
8. Analyze the data according to manufacturer protocol.
** For 3 step cycling protocols, anneal at optimal annealing temperature for 30 sec followed by the minimum time required for data acquisition at 72 ºC according to instrument guidelines.