igScript™ Probe-Based qPCR master mix provides improved PCR efficiency, wider dynamic range, superior sensitivity and specificity.
igScript™ Probe-Based qPCR master mix
$215.00 – $699.00
Description
IgScript™ Probe-Based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low RO X reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust qPCR reactions. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.
Product Components and Storage
- igScript™ Probe-Based qPCR master mix
- –20 °C
Applications
- Gene expression data validation.
- Multiplexing
- Mutation detection
- Pathogen and viral detection
- Genetically modified organisms (GMO) characterization and Genetic profiling
Benefits
- Enhanced efficiency, specificity, and sensitivity
- Compatible with all real-time PCR instruments
- Superior gene expression results under various cycling conditions
Product Use Limitation and Disclaimers
This product is for research purposes only. It is not intended for therapeutic or diagnostic purposes in humans or
animals. This product contains chemicals which may be harmful if misused or direct human contact is made.
Intact Genomics is dedicated to practicing and maintaining science and technology ethics. Buyer agrees to use
the purchased materials in full compliance with applicable law and regulations.
Technical Support
Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.
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Additional information
| reaction | 500 reactions (20 µl rxn vol), 1000 reactions (20 µl rxn vol), 2500 reactions (20 µl rxn vol) |
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Protocol
- Place kit components and DNA samples on ice.
- Mix and then centrifuge briefly to collect contents at the bottom of the tube.
- Prepare a master mix for each reaction and control requiring Reverse Transcriptase enzyme plus 10% extra to allow for pipetting error according to the following table:
- Mix the reaction mixture thoroughly.
- Program the thermal cycler according to the manufacturer’s instructions.
- A typical PCR cycling program is outlined in the following table.
- Place the PCR tubes in the thermal cycler and start the cycling program.
- Analyze the data according to manufacturer protocol.
* For 3 step cycling protocols, anneal at optimal annealing temperature for 30 sec followed by the minimum time required for data acquisition at 72 ºC according to instrument guidelines.
