A box of 3 0 mg / ml injectable chromatography

Hot Start Taq 2x Master Mix

Name: Hot Start Taq 2x Master Mix
SKU: 1758
Availability: InStock

$210.00

Description

Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl₂ and stabilizers with optimized reaction buffer. Intact Genomics Hot Start Taq 2x Master Mix has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´-flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with the unique Intact Genomics 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.

Applications

Routine PCR and RT-PCR
Primer extension
Colony PCR
Genotyping
Efficient for amplifying high GC content template DNA with Magic Enhancer.

Product Includes

Hot start Taq 2x master mix
5X Magic Enhancer

1x Master Mix Composition

10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl_2,0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Hot start Taq Polymerase.

References:
1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. 127, 1550-1557.
2. Lawyer, F.C. et al. (1993). PCR Methods and Appl. 2, 275-287.
3. Longley, M.J., Bennett, S.E and Mosbaugh D.W. Nucleic Acids Res.18, 7317-7322.
4. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.

Additional information

reaction	100 reactions (50 μl rxn vol), 500 reactions (50 μl rxn vol), 100 reactions (20 μl rxn vol), 500 reactions (20 μl rxn vol)

Protocol

1. Prepare a reaction mix according to the following table:

1. 1. Mix the reaction mixture thoroughly.
  2. Program the thermal cycler according to the manufacturer’s instructions.
  3. A typical PCR cycling program is outlined in the following table.

1. 1. Place the PCR tubes in the thermal cycler and start the cycling program.
  2. Analyze 5 μl of PCR products by agarose gel electrophoresis.

References:

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. 127, 1550-1557.
2. Lawyer, F.C. et al. (1993). PCR Methods and Appl. 2, 275-287.
3. Longley, M.J., Bennett, S.E and Mosbaugh D.W. (1990) Nucleic Acids Res.18, 7317-7322.
4. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.