Competent Cells
At Intact Genomics, we strive to provide competent cells that display the highest transformation efficiency, consistency and best value on the market. Utilizing our scientists’ expertise, state of the art equipment and advanced protocols, our competent cells are manufactured to provide transformation efficiency that is often 5-10X that of the competition. Our competent cells are available in a variety of strains including: DH5-Alpha competent cells (ig™ 5-alpha), 10-Beta competent cells (ig™ 10B), and BL21 (DE3) competent cells. Electroporation or chemically competent cells available. We can work with you to find a size and order option that works best for your research needs as well. We also can provide custom competent cells to meet your specific needs. Intact Genomics’ competent cells can increase the efficiency of your transformations while saving you lab time and significant research funding.
-
Struggling to express toxic or difficult proteins? Intact Genomics (IG®) C41(DE3) Electocompetent Cells are engineered to improve tolerance to toxic protein expression while maintaining reliable T7-driven performance. Derived from BL21(DE3), C41(DE3) strain reduces cell death associated with membrane proteins, nucleases, and other challenging targets. When standard BL21(DE3) fails, C41(DE3) helps move your project forward. Custom formats, custom package sizes and bulk pricing are available upon request. -
Built for Tough Protein Expression. Designed for IPTG-inducible T7 systems, this BL21(DE3) derivative improves tolerance to toxic proteins while the pLysS plasmid suppresses basal expression for greater stability. Validated in 400+ publications, IG® C41(DE3)pLysS cells deliver dependable results for challenging protein expression. Custom formats, custom package sizes and bulk pricing are available upon request. -
Intact Genomics (IG®) C43(DE3) Chemically Competent Cells are optimized for the expression of toxic and difficult proteins from vectors with an IPTG-inducible T7 promoter. When protein expression gets tough, choose IG C43(DE3) competent cells. Custom formats (8-well strip tube, 96 or 384 well plate), package sizes, and bulk pricing are available upon request. -
Intact Genomics (IG®) C43(DE3) Electrocompetent Cells are optimized for the expression of toxic and difficult proteins from vectors with an IPTG-inducible T7 promoter. When protein expression gets tough, choose IG C43(DE3) competent cells. Custom formats, custom package sizes and bulk pricing are available upon request. -
Reliable Expression for Toxic Proteins. IG® C43(DE3)pLysS Electrocompetent Cells are designed for high-efficiency transformation and stable expression of toxic or difficult proteins in IPTG-inducible T7 systems. Derived from BL21(DE3), this strain improves tolerance to toxic proteins, while the pLysS plasmid suppresses basal expression to stabilize challenging constructs. Custom formats, custom package sizes and bulk pricing are available upon request. -
Accelerate DNA and BAC engineering with IG® Recombineering Chemically Competent Cells. Designed for DNA, plasmid, and BAC engineering, as well as homologous recombination, with high transformation efficiency. IG® Recombineering Chemically Competent Cells supports precise modification using efficient galK positive/negative selection. Custom formats, custom package sizes, and bulk pricing are available upon request. -
Accelerate DNA and BAC engineering with IG® Recombineering Chemically Competent Cells. Designed for DNA, plasmid, and BAC engineering, as well as homologous recombination, with high transformation efficiency. IG® Recombineering Chemically Competent Cells supports precise modification using efficient galK positive/negative selection. Custom formats, custom package sizes, and bulk pricing are available upon request. -
IG® 10B chemically competent cells offer the highest transformation efficiency on the market, making them the gold standard for creating cDNA or genomic libraries and transforming large plasmids (>10 kb). Custom package sizes are available to fit your lab’s specific needs.
-
(ig®) ccdB Resist™ cells are capable of propagation of plasmids containing the ccdB gene. -
High Efficiency ig® ER2738 Phage Display chemically competent cells are suitable for preparation of antibody or peptide phage display libraries. -
High Efficiency ig® Stable 2 cells are capable of cloning methylated genomic sequences, retroviral sequences and direct repeat sequences. -
High Efficiency ig® XL1 Blue Max chemically competent E. coli cells are suitable for the preparation of high quality plasmid DNA and single strand rescue of phagemid DNA. -
JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. Suitable for high efficiency transformation in a wide variety of applications. -
JM109(DE3) contains a chromosomal copy of the gene for T7 RNA polymerase. -
Reliable, high-efficiency transformation for routine cloning applications. IG® 5-alpha chemically competent cells deliver consistent performance and fast recovery, helping streamline everyday workflows. Custom package sizes and bulk pricing are available to fit your lab’s specific needs. -
Staphylococcus aureus RN4220 strain is commonly used in studies involving virulence, resistance, metabolism and more. -
Intact Genomics (IG®) BL21(DE3) Chemically Competent Cells deliver high transformation efficiency and reliable performance for cloning and recombinant protein production. Optimized for T7-based expression systems, they provide consistent results you can trust.
✔ High transformation efficiency
✔ Excellent performance for protein expression
✔ Custom formats available: tubes, 96-well plates, and 384-well plates
✔ Flexible solutions for high-throughput workflows. -
Simplify your large DNA transformation workflow by eliminating time-consuming heat shock, lengthy incubations, and outgrowth procedures. Simply mix and directly plate! -
Engineered for superior transformation of large DNA molecules (≥10 kb up to 350 kb), igMax™ DH10B ElectroCompetent Cells deliver exceptional performance with transformation efficiencies of ≥5 × 10¹⁰ cfu/µg DNA. Ideal for cloning large plasmids, BACs, and other high-molecular-weight constructs. -
BL21(DE3)pLysS chemically competent cells allow for tighter control of expression and are suitable for the expression of toxic genes. -
Struggling to express toxic or difficult proteins? Intact Genomics (IG®) C41(DE3) Chemically Competent Cells are engineered to improve tolerance to toxic protein expression while maintaining reliable T7-driven performance. Derived from BL21(DE3), C41(DE3) strain reduces cell death associated with membrane proteins, nucleases, and other challenging targets. When standard BL21(DE3) fails, C41(DE3) helps move your project forward. Custom formats (8-well strip, 96 or 384 well plate), custom package sizes and bulk pricing are available upon request. -
Built for Tough Protein Expression.
Designed for IPTG-inducible T7 systems, this BL21(DE3) derivative improves tolerance to toxic proteins while the pLysS plasmid suppresses basal expression for greater stability. Validated in 400+ publications, IG® C41(DE3)pLysS cells deliver dependable results for challenging protein expression. Custom formats (8-well strip, 96 or 384 well plate), package sizes and bulk pricing aare available upon request. -
Reliable Expression for Toxic Proteins.
IG® C43(DE3)pLysS Chemically Competent Cells are designed for high-efficiency transformation and stable expression of toxic or difficult proteins in IPTG-inducible T7 systems. Derived from BL21(DE3), this strain improves tolerance to toxic proteins, while the pLysS plasmid suppresses basal expression to stabilize challenging constructs.
Custom formats (8-well strip, 96 or 384 well plate), custom package sizes and bulk pricing are available upon request. -
IG® Autoinduction DE3 Chemically Competent Cells are suitable for transformation and routine recombinant protein expressed from a vector under an IPTG-inducible T7 promoter. With simple and less hands-on autoinduction, you can either enjoy more free-time or get more work done. Moreover, the yields of both expressed cells and protein are likely 5-10 fold or more for a broad range of protein targets at either small or large scale of protein production. -
Intact Genomics (IG®) Autoinduction DE3 ElectroCompetent cells are suitable for transformation and routine recombinant protein expressed from a vector under an IPTG-inducible T7 promoter. With simple and less hands-on autoinduction, you can either enjoy more free-time or get more work done. Moreover, the yields of both expressed cells and protein are likely 5-10 fold or more for a broad range of protein targets at either small or large scale of protein production. -
Intact Genomics (IG®) Autoinduction Medium promotes high-yield growth of E. coli and high-level expression of T7 promoter-regulated recombinant proteins without time-consuming steps such as monitoring optical density (OD) or adding induction components like IPTG. Unlike traditional LB-IPTG induction systems, IG® Autoinduction Medium allows regulated protein expression in any expression system that is solely inducible by IPTG from E. coli strains containing a fully functional lac operon. -
(ig®) ccdB Resist™ ElectroCompetent Cells are capable of propagation of plasmids containing the ccdB gene. -
Intact Genomics ER2738 Phage Display Electrocompetent Cells are ideal for Phage display library screening and panning, antibody and peptide selection, recombinant phage amplification and recovery. -
Stable 2 cells are capable of cloning methylated genomic sequences, retroviral sequences and direct repeat sequences. -
XL1 Blue Max cells provide transformation efficiencies that are significantly higher than any competitor's similar product, allowing for increased opportunity for experimental success. -
Methionine-Auxotrophic EHA105Met Chemically Competent Agrobacterium reduces bacterial overgrowth during co-cultivation -
Methionine-Auxotrophic EHA105Met Agrobacterium ElectroCompetent Cells reduces bacterial overgrowth during co-cultivation -
Methionine-Auxotrophic LBA4404Met Agrobacterium Chemically Competent Cells reduces bacterial overgrowth during co-cultivation -
Methionine-Auxotrophic LBA4404Met Electrocompetent Agrobacterium reduces bacterial overgrowth during co-cultivation -
MG1655 Chemically Competent Cells are made from the "wild type" K-12 strain with minimal genetic manipulation. -
ig® Thymidine-Auxotrophic EHA101 Agro cells include modifications so that they will not grow unless thymidine is added to Minimal medium. This prevents the bacteria from overgrowing plant tissues. -
ig® Thymidine-Auxotrophic EHA101 Agro cells includes modifications so that they will not grow unless thymidine is added to Minimal medium. This prevents the bacteria from overgrowing plant tissues. -
ig® Thymidine-Auxotrophic EHA105 Agro cells includes modifications so that they will not grow unless thymidine is added to Minimal medium. This prevents the bacteria from overgrowing plant tissues. -
ig® Thymidine-Auxotrophic EHA105 Agro cells includes modifications so that they will not grow unless thymidine is added to Minimal medium. This prevents the bacteria from overgrowing plant tissues. -
ig® Thymidine-Auxotrophic EHA105D Agro cells includes modifications so that they will not grow unless thymidine is added to Minimal medium. This prevents the bacteria from overgrowing plant tissues. -
ig® Thymidine-Auxotrophic EHA105D Agro cells includes modifications so that they will not grow unless thymidine is added to Minimal medium. This prevents the bacteria from overgrowing plant tissues. -
The pSoup plasmid aids in replication of pGreen, 62SK, and pGs2 series plasmids. AGL-1 (pSoup) is used for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.
