Description
The Red system of bacteriophage λ consists of three proteins that promote DNA recombination initiated at dsDNA breaks or at the overlapping ends of the linear λ chromosome (1). The Exo gene (redα) encodes λ exonuclease, a 24-kDa protein with 5´→3´ exonuclease activity (2). The bet gene (redβ) encodes the 29-kDa β protein, which binds ssDNA and promotes the annealing of complementary strands (3). The gam gene (redγ) encodes the 16-kDa γ protein, which binds and inhibits host nuclease enzymes (4). This product contains lambda and gamma, as well as 10x Lambda Gamma reaction buffer.
Applications
• Bind to the host RecBCD protein and inhibits host nuclease enzymes
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.
Lambda gamma is free from detectable endonuclease and RNase activities.
Protein purity
The physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).
Source
E. coli cells carrying λ redγ gene.
Contents & Storage
• Lambda Gamma
• 10x Lambda Gamma reaction buffer
Store all contents at -20 °C.
10x Lambda gamma reaction buffer
670 mM Glycine-KOH 25 mM MgCl2 500 μg/ml BSA pH 9.4 @ 25°C.
Storage Buffer
50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM β-mercaptoethanol, 1 mM DTT and 50% (v/v) glycerol.
Inactivation
Inactivated by heating at 70°C for 20 min.
References
1. Poteete AR. FEMS Microbiol Lett 2001; 201:9 -14.
2. Little, J.W. (1981). Gene Ampli. Anal. 2, 135-145.
3. Kmiec E, Holloman WK. J Biol Chem 1981; 256:12636 -12639.
4. Marsic N, Roje S, Stojiljkovic I, Salaj-Smic E, Trgovcevic Z. J Bacteriol 1993;175:4738 -4743.
Technical Support
Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.
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