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Custom Competent Cells

Intact Genomics offers high-quality custom competent cell services. With a commitment to excellence and tailored solutions, we are here to empower your research and innovation endeavors. Intact Genomics’ custom competent cell services include the following key features and benefits:

  • Customization – We understand that different research projects have unique requirements. Intact Genomics offers customization options to meet specific demands, such as the choice of bacterial strains, vectors, or additional modifications.
  • High Transformation Efficiency – We developed proprietary methods that ensure cells provide the highest efficiency.
  • Quality Control – We maintain rigorous quality control (ISO 13485:2016) measures to guarantee the reliability and performance of all custom competent cells.
  • Format Flexibility – You choose the aliquot size, tube, or 96 well plate.
  • Save Time – Let us take the time to make your cells provide superb results.
  • Affordable – We provide an affordable price in the market to save your funds.
  • Quick Turnaround – Receive your strain, optimize transformation and provide results within 1-2 weeks.
  • Expert Support – Our scientists are the premier experts in competent cell production, cloning and transformation.
  • Excellent Customer Service – Personalized service from start to finish. Our sales and customer service team responds to customer inquiries quickly and accurately.

To get started, please click the Custom Competent Cells Service Form.

Call 855.835.7172 or e-mail sales@intactgenomics.com to discuss your project.

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Scientific equipment and packaging assortment

Product Description

The scientists at Intact Genomics are the premier experts in competent cell production, with unmatched quality, service and the greatest value in the industry. IG custom competent cells are available in chemically competent or electrocompetent format.  You may choose from a variety of common E. coli strains (5-alpha, DH10B, BL21(DE3), etc., or you may simply send us your own strain. Upon request, we are also able to safely store your strain to make production of additional competent cells at a later date convenient and fast.

Intact Genomics uses proprietary methods and technologies to produce the highest transformation efficiencies possible (> 5 x 10^10 cfu/µg DNA).  Using our unique production methods, we have significant experience providing custom competent cells for multiple customers in the pharmaceutical, agricultural and biotech industries allowing significant progress with their research.

Applications:

• Cloning and other genetic engineering applications
• Phage display (TG1 and SS320  strains available)
• Protein expression
• Simplified closing and transformation – custom DirectPlate® competent cells available.
• Library construction

Customers choose our custom competent cell services as we provide great results, value, convenience, and excellent customer service.

To get started, please click the Custom Competent Cells Service Form.

Technical Support

Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.

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Intact Genomics Custom Competent Cell Service Form

  • Please fill out the Custom Competent Cell Service Form below
  • Email the form and any questions to sales@intactgenomics.com
  • Our team will thoroughly review the form and reply within one business day
  • Choose your strain from our list of available strains, or your own, and mail to:
      • Intact Genomics
      11840 Westline Industrial Drive, Suite 120, St. Louis, MO 63146
  • We will notify you of strain receipt and expected completion time (typically within one week)

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    Variations Products Quantity Price Buy Link
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    IG® Recombinase Polymerase Amplification (RPA) Kit V2
    New improved IG® Recombinase Polymerase Amplification (RPA) Kit v2 provides a mixture of the necessary enzymes to simplify and quicken the setup for your RPA reaction.
    Price range: $243.00 through $2,125.00
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    FastAmp® qRPA SYBR Kit
    FastAmp® qRPA SYBR Kit is a powerful tool to screen primer conditions for the lowest possible Non-template control (NTC) background. Applications and Benefits:
    • Primer validation and screening.
    • Highly selective and sensitive isothermal amplification technique.
    • Alternative to PCR. No thermocycler needed.
    • Speed and sensitivity.
    • No DNA pretreatment required.
    • Flexible endpoint detection compatibility (e.g. lateral flow, real-time fluorescence).
    Price range: $290.00 through $2,450.00
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    DirectPlate™ 10B Chemically Competent Cells
    Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ 10B chemically competent E. coli cells provide higher transformation efficiency than any competitor’s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. Our DirectPlate™ 10B chemically competent cells have the highest transformation efficiency on the market.
    Price range: $160.00 through $355.00
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    DirectPlate™ TG1 Phage Display Chemically Competent Cells
    Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ TG1 Phage Display chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ TG1 Phage Display chemically competent E. coli cells provide higher transformation efficiency than any competitor’s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning, phage display libraries and sub-cloning.
    Price range: $155.00 through $335.00
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    FastAmp® Plant Direct RPA Kit
    Intact Genomics FastAmp® Plant Direct RPA kit offers a fast, robust method for amplifying DNA fragments from plant tissues at a single and constant temperature (37°C) using RPA technology. The key highlights of FastAmp® Plant Direct RPA Solution include its simplicity, speed, and reliability. By eliminating the need for DNA extraction, researchers can streamline their workflows, with tissue lysis and genomic DNA ready for RPA in just 3 minutes.
    Price range: $285.00 through $2,255.00
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    IG® Recombinase Polymerase Amplification (RPA) Kit V1
    IG® Recombinase Polymerase Amplification (RPA) Kit provides a mixture of the necessary enzymes to simplify and quicken the setup for your RPA reaction.
    Price range: $230.00 through $1,670.00
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    T4 UvsX DNA Recombinase
    T4 UvsX DNA Recombinase is useful for Recombinase Polymerase Amplification (RPA) and other applications.
    Price range: $235.00 through $1,185.00
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    T4 UvsY Protein
    T4 UvsY efficiently promotes the UvsX-catalyzed strand invasion reaction by recruiting to ssDNA–gp32 complexes, promotes the release of gp32, and favors the binding of UvsX.
    Price range: $255.00 through $1,345.00
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    T4 gp32 Protein
    T4 gp32 Protein is useful for many applications including PCR and RPA (Recombinase Polymerase Amplification)
    Price range: $65.00 through $465.00
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    Bsu DNA Polymerase Large Fragment
    Bsu DNA Polymerase I, Large Fragment is suitable for various purposes including RPA (Recombinase Polymerase Amplification)
    Price range: $55.00 through $305.00
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    Sau DNA Polymerase Large Fragment
    Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.
    Price range: $115.00 through $395.00
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    DirectPlate™ DH5-Alpha Chemically Competent Cells
    Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ DH5-Alpha chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ DH5-Alpha chemically competent E. coli cells provide higher transformation efficiency than any competitor’s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.
    Price range: $165.00 through $1,025.00
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    IG® RPA Master Mix
    Fast, reliable, and easy-to-use — the IG RPA Master Mix (patent pending) delivers powerful isothermal amplification for your molecular diagnostics and research.
    • Works at 25–37°C — no thermal cycler needed
    • More affordable and accessible
    • Optimized for high sensitivity and consistent results
    • Simplify your workflow. Amplify your results.
    Price range: $149.00 through $1,668.00
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    DirectPlate™ BL21(DE3) Chemically Competent Cells
    Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ BL21(DE3) chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ BL21(DE3) chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning, protein expression and sub-cloning.
    Price range: $170.00 through $386.00
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    Cas9 Nuclease
    Intact Genomics Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS).
    Price range: $98.00 through $510.00
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    T4 UvsX DNA Recombinase, Glycerol-Free or Lyophilized
    Glycerol Free T4 UvsX DNA Recombinase is useful for Recombinase Polymerase Amplification (RPA) and other applications
    Price range: $250.00 through $1,420.00
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    DirectPlate XL™ DH10B Chemically Competent Cells
    Simplify your large DNA transformation workflow by eliminating time-consuming heat shock, lengthy incubations, and outgrowth procedures. Simply mix and directly plate!
    $397.00
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    Cas12a Nuclease
    Intact Genomics Cas12a Nuclease is the purified recombinant Francisella tularensis Cas12a enzyme for in vitro gene editing.
    Price range: $101.00 through $541.00
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    PEmax Enzyme
    IG® PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-MMLV-Reverse Transcriptase fusion protein containing a NLS.
    Price range: $276.00 through $500.00
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    T4 UvsY Protein, Glycerol-Free or Lyophilized
    Glycerol Free T4 UvsY efficiently promotes the UvsX-catalyzed strand invasion reaction by recruiting to ssDNA–gp32 complexes, promotes the release of gp32, and favors the binding of UvsX.
    Price range: $270.00 through $1,580.00
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    T4 gp32 Protein, Glycerol-Free or Lyophilized
    Glycerol Free or Lyophilized T4 gp32 Protein is useful for many applications including PCR and RPA (Recombinase Polymerase Amplification).
    Price range: $170.00 through $570.00
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    T7 RNA Polymerase
    T7 RNA Polymerase is useful for sgRNA and mRNA synthesis, RNA synthesis from DNA templates and applications in mRNA therapeutics.
    Price range: $74.00 through $283.00
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    Bsu DNA Polymerase Large Fragment, Glycerol-Free or Lyophilized
    Glycerol Free or Lyophilized Bsu DNA Polymerase I, Large Fragment is suitable for various purposes including RPA (Recombinase Polymerase Amplification).
    Price range: $85.00 through $410.00
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    IG® sgRNA Synthesis Kit for Cas9 Nuclease
    With high and scalable yield, the IG® sgRNA Synthesis Kit for Cas9 nuclease is the perfect choice for a variety of sgRNA synthesis needs.
    Price range: $226.00 through $1,153.00
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    Endonuclease IV (Nfo)
    Endonuclease IV (Nfo) from Escherichia coli is a 32-kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4). Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radiation (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).
    Price range: $150.00 through $295.00
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    IG® sgRNA Synthesis Kit for Cas12a Nuclease
    With high and scalable yield, the IG® sgRNA Synthesis Kit for Cas12a nuclease is the perfect choice for a variety of sgRNA synthesis needs
    Price range: $246.00 through $1,183.00
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    IG® PE pegRNA Synthesis Kit
    The IG® PE pegRNA synthesis kit is simple to use, and is scalable to help you reach necessary yields of pegRNA
    Price range: $370.00 through $1,643.00
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    T7 Endonuclease I
    T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits (1) is the product of a recombinant gene in E. coli . Mutations generated by CRISPR/ZFN/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. If the mismatched DNA length position is more than 1 bp, T7 Endonuclease I can recognize and cleave it. It is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells.
    Price range: $98.00 through $210.00
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    IG® 10B Electrocompetent Cells
    Intact Genomics ig® 10B (DH10B derivative) electrocompetent E. coli cells offer the highest transformation efficiencies of ≥5 x 1010 cfu/µg plasmid DNA which are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.
    Price range: $139.00 through $386.00
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    IG® XL1 Blue Max Electrocompetent E. coli Cells
    XL1 Blue Max cells provide transformation efficiencies that are significantly higher than any competitor’s similar product, allowing for increased opportunity for experimental success.
    Price range: $139.00 through $263.00
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    IG® 10B Chemically Competent Cells (High Efficiency)
    IG® 10B chemically competent cells offer the highest transformation efficiency on the market, making them the gold standard for creating cDNA or genomic libraries and transforming large plasmids (>10 kb). Custom package sizes are available to fit your lab’s specific needs.
    Price range: $98.00 through $998.00
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    IG C41(DE3) Electrocompetent Cells
    Struggling to express toxic or difficult proteins? Intact Genomics (IG®) C41(DE3) Electocompetent Cells are engineered to improve tolerance to toxic protein expression while maintaining reliable T7-driven performance. Derived from BL21(DE3), C41(DE3) strain reduces cell death associated with membrane proteins, nucleases, and other challenging targets. When standard BL21(DE3) fails, C41(DE3) helps move your project forward. Custom formats, custom package sizes and bulk pricing are available upon request.
    Price range: $295.00 through $785.00
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    IG® XL1 Blue Max Chemically Competent Cells
    High Efficiency ig® XL1 Blue Max chemically competent E. coli cells are suitable for the preparation of high quality plasmid DNA and single strand rescue of phagemid DNA.
    Price range: $139.00 through $232.00
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    IG C41(DE3)pLysS Electrocompetent Cells
    Built for Tough Protein Expression. Designed for IPTG-inducible T7 systems, this BL21(DE3) derivative improves tolerance to toxic proteins while the pLysS plasmid suppresses basal expression for greater stability. Validated in 400+ publications, IG® C41(DE3)pLysS cells deliver dependable results for challenging protein expression. Custom formats, custom package sizes and bulk pricing are available upon request.
    Price range: $300.00 through $795.00
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    HB101 Chemically Competent Cells
    Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening.
    Price range: $125.00 through $324.00
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    IG® Autoinduction Medium
    Intact Genomics (IG®) Autoinduction Medium promotes high-yield growth of E. coli and high-level expression of T7 promoter-regulated recombinant proteins without time-consuming steps such as monitoring optical density (OD) or adding induction components like IPTG. Unlike traditional LB-IPTG induction systems, IG® Autoinduction Medium allows regulated protein expression in any expression system that is solely inducible by IPTG from E. coli strains containing a fully functional lac operon.
    Price range: $85.00 through $525.00
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    JM109(DE3) Chemically Competent Cells
    JM109(DE3) contains a chromosomal copy of the gene for T7 RNA polymerase.
    Price range: $129.00 through $273.00
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    JM109 Chemically Competent Cells
    JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. Suitable for high efficiency transformation in a wide variety of applications.
    Price range: $124.00 through $273.00
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    Glycerol-Free RNase Inhibitor, Murine
    Intact Genomics (ig®) Glycerol-free RNase Inhibitor is a powerful tool to inhibit and control RNase contaminants in enzymatic manipulations of RNA. It is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C.
    Price range: $170.00 through $381.00
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    RNase Inhibitor, Murine
    Intact Genomics RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C
    Price range: $118.00 through $294.00
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    Tth DNA Ligase

    High-fidelity Thermus thermophilus (Tth) DNA Ligase from Intact Genomics delivers precise, thermostable ligation with excellent SNP discrimination and low background—ideal for LCR and mutation detection.

    Custom formats, package sizes and bulk pricing are available upon request.
    Price range: $82.00 through $395.00
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    igScript™ Glycerol-free Reverse Transcriptase
    igScript™ Glycerol-free Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates.
    Price range: $160.00 through $366.00
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    MG1655 Chemically Competent Cells
    MG1655 Chemically Competent Cells are made from the “wild type” K-12 strain with minimal genetic manipulation.
    Price range: $201.00 through $407.00
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    igScript™ Reverse Transcriptase
    igScript™ Reverse Transcriptase (igScript RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. igScript RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.
    Price range: $108.00 through $283.00
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    igScript™ First Strand cDNA Synthesis Kit
    igScript™ first strand cDNA synthesis kit includes 5x igScript™ master mix which contains igScript™ Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl2 and protein stabilizers. igScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nuclease-free water. An anchored Oligo-dT primer forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-55 ºC.
    Price range: $124.00 through $391.00
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    IG® Stable 2 Chemically Competent Cells
    High Efficiency ig® Stable 2 cells are capable of cloning methylated genomic sequences, retroviral sequences and direct repeat sequences.
    Price range: $134.00 through $232.00
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    IG C43(DE3) Electrocompetent Cells
    Intact Genomics (IG®) C43(DE3) Electrocompetent Cells are optimized for the expression of toxic and difficult proteins from vectors with an IPTG-inducible T7 promoter. When protein expression gets tough, choose IG C43(DE3) competent cells. Custom formats, custom package sizes and bulk pricing are available upon request.
    Price range: $295.00 through $785.00
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    igScript™ One Step RT-PCR Kit
    igScript™ One Step RT-PCR kit combines two powerful mixtures: i) igScript™ Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.
    Price range: $185.00 through $700.00
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    igScript™ One Step RT-qPCR Kit
    igScript™ One Step RT-qPCR kit combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.
    Price range: $201.00 through $716.00
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    IG Recombineering Chemically Competent Cells
    Accelerate DNA and BAC engineering with IG® Recombineering Chemically Competent Cells. Designed for DNA, plasmid, and BAC engineering, as well as homologous recombination, with high transformation efficiency. IG® Recombineering Chemically Competent Cells supports precise modification using efficient galK positive/negative selection. Custom formats, custom package sizes, and bulk pricing are available upon request.
    Price range: $155.00 through $475.00
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    IG C43(DE3)pLysS Electrocompetent Cells
    Reliable Expression for Toxic Proteins. IG® C43(DE3)pLysS Electrocompetent Cells are designed for high-efficiency transformation and stable expression of toxic or difficult proteins in IPTG-inducible T7 systems. Derived from BL21(DE3), this strain improves tolerance to toxic proteins, while the pLysS plasmid suppresses basal expression to stabilize challenging constructs. Custom formats, custom package sizes and bulk pricing are available upon request.
    Price range: $300.00 through $795.00
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    igScript™ Probe-Based One Step RT-qPCR Kit
    IgScript™ Probe-Based One Step RT-qPCR 2x master mix contains IgScript™ Reverse Transcriptase, Taq DNA polymerase, MgCl2, dNTPs, stabilizers and low ROX reference dye with standard buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ Probe-Based One Step RT-qPCR Kit  is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in RT-qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.
    Price range: $201.00 through $757.00
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    IG® ccdB Resist™ Chemically Competent Cells
    (ig®) ccdB Resist™ cells are capable of propagation of plasmids containing the ccdB gene.
    Price range: $149.00 through $237.00
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    igScript™ Probe-Based RT-qPCR Kit
    igScript™ Probe-Based qPCR master mix provides improved PCR efficiency, wider dynamic range, superior sensitivity and specificity.
    Price range: $205.00 through $1,441.00
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    TG1 Phage Display Electrocompetent Cells
    Intact Genomics TG1 Phage Display ElectroCompetent Cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.
    Price range: $191.00 through $438.00
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    Taq DNA Ligase
    Intact Genomics Taq DNA Ligase delivers exceptional specificity and performance for ligation-based PCR, SNP detection, and molecular diagnostics. Achieve precise, high-temperature DNA ligation. Perfect for applications demanding accuracy and reliability.
    Price range: $85.00 through $250.00
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    Ar.A4 Agrobacterium Chemically Competent Cells
    Transformation Protocol Use this procedure to transform Ar.A4 Chemically Competent Agrobacterium cells. Do not use these cells for electrocompetent transformation.
    1. Place microcentrifuge tubes on ice.
    2. Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
    3. Aliquot 1 µl (10pg -1 µg) of DNA to the chilled microcentrifuge tubes on ice.
    4. When the cells are thawed, add 50μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pIG7spe control, add 1 µl of (500 pg/µl) DNA to the 50 µl of cells on ice. Mix well by tapping. Do not pipette up and down or vortex to mix, this can harm cells and decrease transformation efficiency.
    5. Keep tubes on ice for 5 minutes, and then transfer to liquid nitrogen for 5 minutes.
    6. Incubate tubes for additional 5 minutes in 37°C water bath.
    7. Immediately add 950µl of Recovery Medium or any other medium of choice to the tube, pipette up and down three times to re-suspend the cells.
    8. Incubate tubes at 30 °C for 3 hours at 200 RPM.
    9. Dilute the cells as appropriate then spread 20-200 μl cells onto a pre-warmed selective plate. For the pIG7spe control, you may plate 100 μl of undiluted transformation mix onto a YT plate containing 100 μg/ml spectinomycin. Use a sterilized spreader or autoclaved ColiRoller™ plating beads to spread evenly.
    10. Incubate the plates for 2 – 3 days at 30 °C.
    Price range: $335.00 through $747.00
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    IG® Autoinduction DE3 Chemically Competent Cells
    IG® Autoinduction DE3 Chemically Competent Cells are suitable for transformation and routine recombinant protein expressed from a vector under an IPTG-inducible T7 promoter. With simple and less hands-on autoinduction, you can either enjoy more free-time or get more work done. Moreover, the yields of both expressed cells and protein are likely 5-10 fold or more for a broad range of protein targets at either small or large scale of protein production.
    Price range: $115.00 through $1,150.00
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    SS320 Phage Display Electrocompetent Cells
    Intact Genomics SS320 (MC1061F\’) phage display electrocompetent cells are suitable for protein expression, general cloning, blue/white screening, M13 phage work and phage display protein expression.
    Price range: $201.00 through $448.00
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    Custom Competent Cells
    Intact Genomics offers high-quality custom competent cell services. With a commitment to excellence and tailored solutions, we are here to empower your research and innovation endeavors. Intact Genomics’ custom competent cell services include the following key features and benefits:
    • Customization – We understand that different research projects have unique requirements. Intact Genomics offers customization options to meet specific demands, such as the choice of bacterial strains, vectors, or additional modifications.
    • High Transformation Efficiency – We developed proprietary methods that ensure cells provide the highest efficiency.
    • Quality Control – We maintain rigorous quality control (ISO 13485:2016) measures to guarantee the reliability and performance of all custom competent cells.
    • Format Flexibility – You choose the aliquot size, tube, or 96 well plate.
    • Save Time – Let us take the time to make your cells provide superb results.
    • Affordable – We provide an affordable price in the market to save your funds.
    • Quick Turnaround – Receive your strain, optimize transformation and provide results within 1-2 weeks.
    • Expert Support – Our scientists are the premier experts in competent cell production, cloning and transformation.
    • Excellent Customer Service – Personalized service from start to finish. Our sales and customer service team responds to customer inquiries quickly and accurately.
    To get started, please click the Custom Competent Cells Service Form.

    Call 855.835.7172 or e-mail sales@intactgenomics.com to discuss your project.
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    Ar.A4 Agrobacterium ElectroCompetent Cells
    The Ar.A4 strain is useful for transgenic operations of useful for transgenic operations of corn, tobacco, carrot and other plants.
    Price range: $366.00 through $778.00
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    BL21(DE3) Chemically Competent Cells
    Intact Genomics (IG®) BL21(DE3) Chemically Competent Cells deliver high transformation efficiency and reliable performance for cloning and recombinant protein production. Optimized for T7-based expression systems, they provide consistent results you can trust. ✔ High transformation efficiency ✔ Excellent performance for protein expression ✔ Custom formats available: tubes, 96-well plates, and 384-well plates ✔ Flexible solutions for high-throughput workflows.
    Price range: $101.00 through $1,025.00
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    ATCC15834 Agrobacterium Chemically Competent Cells
    Useful for transgenic operations of legumes, tobacco, variety of grasses and other plants.
    Price range: $366.00 through $778.00
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    T4 DNA Ligase
    Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA.  This enzyme joins DNA fragments with either cohesive or blunt termini and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).
    Price range: $201.00 through $335.00
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    IG® ER2738 Phage Display ElectroCompetent Cells
    Intact Genomics ER2738 Phage Display Electrocompetent Cells are ideal for Phage display library screening and panning, antibody and peptide selection, recombinant phage amplification and recovery.
    Price range: $181.00 through $428.00
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    ATCC15834 Agrobacterium ElectroCompetent Cells
    The ATCC15834 strain is useful for transgenic operations of useful for transgenic operations of legumes, tobacco, variety of grasses and other plants.
    Price range: $366.00 through $778.00
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    IGMax™ DH10B ElectroCompetent Cells
    Engineered for superior transformation of large DNA molecules (≥10 kb up to 350 kb), igMax™ DH10B ElectroCompetent Cells deliver exceptional performance with transformation efficiencies of ≥5 × 10¹⁰ cfu/µg DNA. Ideal for cloning large plasmids, BACs, and other high-molecular-weight constructs.
    Price range: $395.00 through $715.00
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    T4 Polynucleotide Kinase (PNK)
    T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5’-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3’-monophosphates. In the presence of ADP, T4 PNK exhibits 5’-phosphatase activity and catalyzes the exchange of phosphate group between 5’-P-oligonucleotides/polynucleotides and ATP (1). The enzyme is also a 3’-phosphatase (2). 
    $191.00
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    K599 Agrobacterium Chemically Competent Cells
    Useful for transgenic operations of corn, soybean (wild soybean), cotton, peanut, dandelion, cowpea and other plants.
    Price range: $335.00 through $747.00
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    ig® 5 alpha Electrocompetent Cells
    Intact Genomics 5 alpha Electrocompetent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning. Mutations in endA1 and recA1 ensure increased plasmid yield and improved plasmid quality.
    Price range: $149.00 through $402.00
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    BL21 Chemically Competent Cells
    Intact Genomics BL21 chemically competent cells are suitable for transformation and routine protein expression from non-T7 vectors.
    Price range: $98.00 through $196.00
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    BL21(DE3)pLysS Chemically Competent Cells
    BL21(DE3)pLysS chemically competent cells allow for tighter control of expression and are suitable for the expression of toxic genes.
    Price range: $144.00 through $283.00
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    T4 DNA Polymerase
    IG™ T4 DNA Polymerase has both a DNA-dependent DNA polymerase activity and a potent 3´→5´ exonuclease activity. 
    $180.00
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    IG Recombineering Electrocompetent Cells
    Accelerate DNA and BAC engineering with IG® Recombineering Chemically Competent Cells. Designed for DNA, plasmid, and BAC engineering, as well as homologous recombination, with high transformation efficiency. IG® Recombineering Chemically Competent Cells supports precise modification using efficient galK positive/negative selection. Custom formats, custom package sizes, and bulk pricing are available upon request.
    Price range: $245.00 through $765.00
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    IG C41(DE3) Chemically Competent Cells
    Struggling to express toxic or difficult proteins? Intact Genomics (IG®) C41(DE3) Chemically Competent Cells are engineered to improve tolerance to toxic protein expression while maintaining reliable T7-driven performance. Derived from BL21(DE3), C41(DE3) strain reduces cell death associated with membrane proteins, nucleases, and other challenging targets. When standard BL21(DE3) fails, C41(DE3) helps move your project forward. Custom formats (8-well strip, 96 or 384 well plate), custom package sizes and bulk pricing are available upon request.
    Price range: $145.00 through $475.00
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    IG® Stable 2 Electrocompetent E. coli Cells
    Stable 2 cells are capable of cloning methylated genomic sequences, retroviral sequences and direct repeat sequences.
    Price range: $149.00 through $273.00
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    IG® ccdB Resist™ Electrocompetent E. coli Cells
    (ig®)ccdB Resist™ ElectroCompetent Cells are capable of propagation of plasmids containing the ccdB gene.
    Price range: $170.00 through $335.00
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    GV3101 Agrobacterium Chemically Competent Cells
    Useful for Agrobacterium­-mediated genetic transformation of Arabidopsis thaliana, tobacco, corn, potatoes, and other plants.
    Price range: $195.00 through $541.00
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    Staphylococcus Aureus (S. aureus) RN4220 Electrocompetent Cells
    Staphylococcus aureus RN4220 strain is commonly used in studies involving virulence, resistance, metabolism and more.
    Price range: $395.00 through $726.00
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    IG® Autoinduction DE3 ElectroCompetent Cells
    Intact Genomics (IG®) Autoinduction DE3 ElectroCompetent cells are suitable for transformation and routine recombinant protein expressed from a vector under an IPTG-inducible T7 promoter. With simple and less hands-on autoinduction, you can either enjoy more free-time or get more work done. Moreover, the yields of both expressed cells and protein are likely 5-10 fold or more for a broad range of protein targets at either small or large scale of protein production.
    Price range: $230.00 through $425.00
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    GV3101 Agrobacterium ElectroCompetent Cells
    Useful for Agrobacterium­-mediated genetic transformation of Arabidopsis thaliana, tobacco, corn, potatoes, and other plants.
    Price range: $205.00 through $565.00
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    IG C41(DE3)pLysS Chemically Competent Cells
    Built for Tough Protein Expression.
    Designed for IPTG-inducible T7 systems, this BL21(DE3) derivative improves tolerance to toxic proteins while the pLysS plasmid suppresses basal expression for greater stability. Validated in 400+ publications, IG® C41(DE3)pLysS cells deliver dependable results for challenging protein expression. Custom formats (8-well strip, 96 or 384 well plate), package sizes and bulk pricing aare available upon request.
    Price range: $150.00 through $480.00
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    BL21(DE3) Electrocompetent Cells
    Intact Genomics BL21 (DE3) electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21(DE3) electrocompetent E. coli cells ideal for construction of complex expression libraries.
    Price range: $232.00 through $412.00
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    AGL1 Agrobacterium Chemically Competent Cells
    AGL1 Chemically Competent Cells allow for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.
    Price range: $204.00 through $541.00
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    IG® RNA Cleanup Kit
    The IG® RNA Cleanup Kit purifies and concentrates up to 50 µg of RNA from enzymatic reactions.
    Price range: $80.00 through $321.00
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    BL21(DE3)pLysS ElectroCompetent Cells
    BL21(DE3)pLysS electrocompetent cells allow for tighter control of expression and are therefore suitable for the expression of toxic genes.
    Price range: $298.00 through $520.00
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    IG C43(DE3) Chemically Competent Cells
    Intact Genomics (IG®) C43(DE3) Chemically Competent Cells are optimized for the expression of toxic and difficult proteins from vectors with an IPTG-inducible T7 promoter. When protein expression gets tough, choose IG C43(DE3) competent cells. Custom formats (8-well strip tube, 96 or 384 well plate), package sizes, and bulk pricing are available upon request.
    Price range: $145.00 through $475.00
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    AGL1 Agrobacterium ElectroCompetent Cells
    AGL1 electroporation with a binary vector allows for subsequent Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.
    Price range: $205.00 through $565.00
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    IG® 5 Alpha Chemically Competent Cells (High Efficiency)
    Reliable, high-efficiency transformation for routine cloning applications. IG® 5-alpha chemically competent cells deliver consistent performance and fast recovery, helping streamline everyday workflows. Custom package sizes and bulk pricing are available to fit your lab’s specific needs.
    Price range: $99.00 through $998.00
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    DL39(DE3) Chemically Competent Cells
    Intact Genomics chemically competent DL39(DE3) E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label :e.g. phenylalanine or leucine residues.  DL39(DE3) can also be used to reduce NMR cross-labeling via transaminase activity for valine, leucine, isoleucine, aspartate, phenylalanine, tyrosine and tryptophan residues.
    Price range: $145.00 through $465.00
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    LBA4404 Agrobacterium Chemically Competent Cells
    The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants.
    Price range: $204.00 through $541.00
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    IG® ER2738 Phage Display Chemically Competent Cells
    High Efficiency ig® ER2738 Phage Display chemically competent cells are suitable for preparation of antibody or peptide phage display libraries.
    Price range: $160.00 through $268.00
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    LBA4404 Agrobacterium ElectroCompetent Cells
    The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants.
    Price range: $205.00 through $565.00
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    K599 Agrobacterium ElectroCompetent Cells
    IG’s K599 Agrobacterium ElectroCompetent Cells is optimized for the highest transformation efficiencies and is useful for transgenic operations of corn, soybean, cotton, tobacco, carrot, peanut, dandelion, cowpea, and other plants.
    Price range: $361.00 through $773.00
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    Recovery Medium
    Intact Genomics Recovery Medium is for all competent cell transformations.
    Price range: $101.00 through $134.00
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    MSU440 Agrobacterium Chemically Competent Cells
    Useful for transgenic operations of corn, tobacco, wormwood, tea tree and other plants.
    Price range: $366.00 through $778.00
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    EHA105 Agrobacterium Chemically Competent Cells
    The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants.
    Price range: $204.00 through $541.00
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    EHA105 Agrobacterium ElectroCompetent Cells
    The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants.
    Price range: $205.00 through $565.00
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    Quick10™ Cloning Kit
    Quick10™ Cloning Kit enables assembly to plate in 10 Minutes!
    $395.00
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    C58C1 Agrobacterium Chemically Competent Cells
    Useful for transgenic operations that involve potatoes, astragalus, rosaceae, tobacco and other plants.
    Price range: $195.00 through $541.00
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