Description
IG® Biotin Protein Ligase (BirA) is an ATP-dependent enzyme that catalyzes the site-specific covalent attachment of biotin to a specific lysine residue within a compatible biotin-acceptor peptide or protein. The reaction requires biotin, ATP, and magnesium and produces a stable biotinylated protein suitable for downstream detection, immobilization, purification, and interaction studies.
Recombinant Escherichia coli BirA is widely used to biotinylate proteins containing short biotin-acceptor sequences, including AviTag™ (GLNDIFEAQKIEWHE), BioTag (ALNDIFEAQKIEWHA), and BirA Substrate Peptide, or BSP (LHHILDAQKMVWNHR). These peptide sequences can be genetically fused to either the N-terminus or C-terminus of a target protein, enabling specific and controlled biotinylation by BirA.
Compared with nonspecific chemical labeling, BirA-mediated biotinylation provides site-specific modification at a defined lysine residue while helping preserve protein structure and biological activity. Biotinylated proteins bind streptavidin, avidin, or neutravidin with high affinity, supporting sensitive and reproducible applications in protein research, assay development, and biopharmaceutical studies.
Applications:
- In vitro biotinylation of AviTag-, BioTag-, or BSP-tagged proteins
- Preparation of biotinylated proteins for ELISA and other binding assays
- Protein immobilization on streptavidin-coated surfaces
- Protein–protein and protein–ligand interaction studies
- Preparation of biotinylated antigens, antibodies, receptors, and enzymes
- Affinity purification, pull-down assays, and biosensor applications
Benefits:
- Highly specific enzymatic biotinylation
- Controlled labeling at a defined lysine residue
- Helps preserve protein structure and activity
- Produces stable biotinylated proteins with strong streptavidin binding
- Suitable for a wide range of research and assay-development applications
Product Source:
Recombinant E.coli BirA is fused to the C-terminus of maltose-binding protein (MBP). A His-tag is located at the N-terminus of the fusion protein.
Shipping Conditions:
Ship on dry ice or with ice packs.
Specifications:
Purified E.coli BirA fusion protein with a molecular weight of 78 kDa.
Components and Storage Temperature:
The following reagents are supplied with this product:
5031, 5033
