Agrobacterium Antibiotic Resistance and Sensitivity

Gloved hand holding bacterial culture dish

Agrobacterium Antibiotic Sensitivity & Resistance Testing

Collaborators of Intact Genomics have diligently completed the analysis of various Agrobacterium strains for antibiotic sensitivity and resistance to many common antibiotics. As an aid to our customers with their research, we present the below information.

Notes on Experiment:

  • Plates were incubated at 28.5 oC for three days.
  • Tests were performed in LB agar (1.7%) with the appropriate antibiotics.
  • Cells were tested by streaking a loopful of stationary culture grown in 2xYT with 25 µg/ml Rifampicin at 30 oC for 1-2 days.
    Growth with colonies – means that there were good size isolated colonies after three days.
    Growth with no colonies – means that the cells grew but grew very poorly and that the cells are partially sensitive.
    Growth in concentrated area – means that the cells are partially resistant.
    NA- not tested.
  • The strains EHA105 and AGL1 are generally slow growers and patience is required in the experiment.
  • Antibiotics were made fresh and used within 0-2 days (this 2 day experiment used frozen stock from day 0)
Bacterial growth data in green table
Antibiotic growth data table

Agrobacterium Competent Cells

Using Propriety methods, Intact Genomics has developed and manufactured a variety of Agrobacterium chemically and electroporation competent cells with high efficiency. Agrobacterium strains include EHA105, LBA4404, GV3101 and AGL-1. All are useful for plant transgenic operations in a variety of species.

Product Links Efficiency Application & Benefits

GV3101 Agrobacterium Chemically Competent Cells
 ≥1.0 × 105 Transgenic operations of Arabidopsis thaliana, tobacco, corn, potato, and other plants

GV3101 Agrobacterium ElectroCompetent Cells
 ≥1.0 × 107

AGL1 Agrobacterium Chemically Competent Cells
 ≥1.0 × 105 Transgenic operations of Arabidopsis thaliana, maize and other monocots

AGL1 Agrobacterium ElectroCompetent Cells
 ≥1.0 × 107

LBA4404 Agrobacterium Chemically Competent Cells
 ≥1.0 × 105 Transgenic operations of tomatoes, tobacco and other plants

LBA4404 Agrobacterium ElectroCompetent Cells
 ≥1.0 × 107

EHA105 Agrobacterium Chemically Competent Cells
 ≥1.0 × 105 Transgenic operations of rice, tobacco and other plants

EHA105 Agrobacterium ElectroCompetent Cells
 ≥1.0 × 107

C58C1 Agrobacterium Chemically Competent Cells
 ≥1.0 × 105 Transgenic operations involving potatoes, tobacco and other plants

C58C1 Agrobacterium ElectroCompetent Cells
 ≥1.0 × 107

Agrobacterium Chemically Competent Cells Combo Pack
 Various For applications that require multiple Agrobacterium competent cell strains

Agrobacterium ElectroCompetent Cells Combo Pack
 Various

GV3101 (pSoup) Agrobacterium ElectroCompetent Cells
 ≥1.0 × 107 Transgenic operations of Arabidopsis thaliana, tobacco, corn, potato, and other plants

GV3101 (pSoup-p19) Agrobacterium ElectroCompetent Cells
 ≥1.0 × 107 Transgenic operations of Arabidopsis thaliana, tobacco, corn, potato, and other plants

AGL1 (pSoup) Agrobacterium ElectroCompetent Cells
 ≥1.0 × 107 Transgenic operations of Arabidopsis thaliana, maize and other monocots

EHA105 (pSoup) Agrobacterium ElectroCompetent Cells
 ≥1.0 × 107 Transgenic operations of rice, tobacco and other plants