EHA105 (pSoup) Agrobacterium ElectroCompetent Cells

Product Description

Intact Genomics (ig^®) EHA105 (pSoup) Agrobacterium ElectroCompetent Cells are optimized for the highest transformation efficiencies ideal for applications such as cDNA or gDNA library construction. EHA105 (pSoup) contains a rifampicin resistance gene (rif). EHA105 (pSoup) strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which contains the vir gene. The pSoup plasmid aids in replication of pGreen, 62SK, and pGs2 series plasmids. This system is used for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potatoes, and monocots like corn.

Specifications
Competent cell type: Electrocompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Format: Tubes
Transformation efficiency: ≥ 1 x 10⁷ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Shipping condition: Dry ice

Reagents Needed for One Reaction
EHA105 Agrobacterium Electrocompetent Cells: 25 µl
DNA (pCAMBIA1391z, 500 pg/µl): 1 µl
Recovery medium: 1 ml

Storage
EHA105 (pSoup) Agrobacterium ElectroCompetent Cells: -80 ºC
pCAMBIA1391z control DNA: -20 ºC
Recovery medium: 4 ºC

Genotype

C58 (rif^R), Ti pEHA105 (pTiBo542DT-DNA), pSOUP (tet^R), Succinamopine

Quality Control
Transformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ≥1 x 10⁷ CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

Follow these guidelines when using EHA105 (pSoup) Agrobacterium ElectroCompetent Cells:
• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical
lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA,
mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Please note, all agrobacterial strains are not well studied for antibiotic resistance and there are many agrobacterial strains. Therefore, it is the customer’s responsibility to make sure his/her vectors are compatible with the Agrobacterial strains if he/she uses an alternate antibiotic selection than kanamycin-selection.