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Reverse Transcription Enzymes & Kits
Reverse Transcription Solutions
Intact Genomics has developed and manufactured a variety of kits and enzymes useful for various Reverse transcription needs. All kits and products contain Intact Genomics proprietary igScriptâ„¢ Reverse Transcriptase.
| Product Links | Size Available | Application & Benefits |
|---|---|---|
Glycerol-free RNase Inhibitor, Murine |
5,000 & 20,000 Units | A powerful tool to inhibit and control RNase contaminants in enzymatic manipulations of RNA. |
RNase Inhibitor, Murine |
5,000 & 20,000 Units | A powerful tool to inhibit and control RNase contaminants in enzymatic manipulations of RNA. |
igScriptâ„¢ Glycerol-free Reverse Transcriptase |
10,000 & 50,000 Units | A recombinant MMLV reverse transcriptase with reduced RNase H activity and efficient full-length cDNA synthesis from long RNA templates. |
igScriptâ„¢ Reverse Transcriptase |
50,000 Units | A recombinant MMLV reverse transcriptase with reduced RNase H activity and high efficiency for long RNA templates. |
igScriptâ„¢ First Strand cDNA Synthesis Kit |
25 & 100 Reactions | First-strand cDNA synthesis for PCR and RT-PCR applications, active at 55°C. |
igScriptâ„¢ One Step RT-PCR Kit |
100 & 500 Reactions | Combines igScript™ Reverse Transcriptase and Hot Start Taq 2× Master Mix for improved sensitivity, specificity, and PCR efficiency. |
igScriptâ„¢ One Step RT-qPCR Kit |
100 & 500 Reactions | Combines igScript™ Reverse Transcriptase with ig SYBR® Green qPCR 2× Master Mix for one-step RT-qPCR. |
igScriptâ„¢ Probe-Based One Step RT-qPCR Kit |
200, 500, 1,000 & 5,000 Reactions | Single-tube solution for robust probe-based RT-qPCR assays. |
igScriptâ„¢ Probe-Based RT-qPCR Kit |
50, 100 & 500 Reactions | Combines first-strand cDNA synthesis with probe-based qPCR master mix for enhanced PCR performance. |
T7 RNA Polymerase |
5,000U & 25,000U | Ideal for sgRNA and mRNA synthesis, RNA production from DNA templates, and mRNA therapeutic applications. |
igScriptâ„¢ Reverse Transcriptase Related Products
igScript™ Reverse Transcriptase (igScript™ RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. igScript™ RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.
Key Benefits
Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity.
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Active at temperatures up to 55°C.
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Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA.
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Available in many varieties including in our cDNA Synthesis kit (which includes 5x igScriptâ„¢ master mix, Oligo and Hexamer Primers, and nuclease free water)
