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E. coli & Other Electrocompetent Cells
Intact Genomics® Highest Efficiency Electrocompetent E. Coli Cells
Intact Genomics (IG®, ig®) offers the largest collections of high-quality competent cells on the market.
Founded originally as a BAC cloning company, Intact Genomics developed deep expertise in producing ultra-high-efficiency competent cells to enable transformation of very large plasmids (~300 kb) into E. coli. Over the years, this expertise—combined with continuous process optimization—has allowed us to consistently deliver exceptionally high transformation efficiencies.
Leveraging our scientists’ expertise and craftsmanship, state-of-the-art equipment, advanced protocols, and rigorous quality control standards, our competent cells provide the following advantages:
- Highest Transformation Efficiency – Up to 10–100× higher than other leading brands.Quality Control – We maintain rigorous quality control measures in accordance with ISO 13485:2016 to ensure consistent reliability from batch to batch.
- Affordable– Competitive pricing to maximize your research budget.
- Format Flexibility & Custom Services Available – We offer a broader range of formats than competitors, including tubes (multiple aliquot sizes), 96- and 384-well plates, and strip tubes. We also provide custom competent cell services tailored to your specific needs.
Custom Formats, Custom Package Sizes and Bulk Pricing Are Available Upon Request
| Application | Product Link | Specialization | Efficiency |
|---|---|---|---|
| Routine Cloning | ig® 5-alpha Electrocompetent Cells | Routine cloning and sub-cloning, library construction (DNA <10 kb) | ≥2.0 × 1010 |
| ig® 10B Electrocompetent Cells | Routine cloning and sub-cloning, library preparation including large DNA >10 kb | ≥5.0 × 1010 | |
| ig® XL1 Blue Max Electrocompetent Cells | Stable plasmid propagation with blue/white screening | ≥5.0 × 1010 | |
| HB101 Electrocompetent Cells | Stable plasmid maintenance | ≥5.0 × 1010 | |
| Specialized Cloning | igMax™ DH10B Electrocompetent Cells | Difficult large DNA fragment cloning, BAC library construction | ≥5.0 × 1010 |
| IG® Recombineering Electrocompetent Cells | Homologous recombination, DNA, plasmid, and BAC engineering | ≥1.0 × 1010 | |
| ig® Stable 2 Electrocompetent E. coli Cells | Cloning direct repeat sequences (unstable DNA) | ≥2.0 × 109 | |
| ig® ccdB Resist™ Electrocompetent E. coli Cells | Propagation of plasmids containing the ccdB gene | ≥1.0 × 1010 | |
| Staphylococcus aureus (S. aureus) RN4220 ElectroCompetent Cells | A gateway strain to enable DNA manipulation in Staphylococcus aureus | ≥1 × 105 | |
| Protein Expression | IG® Autoinduction DE3 ElectroCompetent Cells | Optimized for autoinduction of protein expression | ≥1.0 × 1010 |
| BL21(DE3) Electrocompetent Cells | Routine T7 protein expression | ≥1.0 × 1010 | |
| BL21(DE3)pLysS ElectroCompetent Cells | Routine non-T7 protein expression | ≥1.0 × 1010 | |
| IG® C41(DE3) Electrocompetent Cells | Improved expression of toxic proteins | ≥1.0 × 1010 | |
| IG® C41(DE3)pLysS Electrocompetent Cells | Improved expression of toxic proteins with suppressed basal expression | ≥1.0 × 109 | |
| IG® C43(DE3) Electrocompetent Cells | Most tolerant of toxic proteins | ≥1.0 × 1010 | |
| IG® C43(DE3)pLysS Electrocompetent Cells | Most tolerant of toxic proteins with suppressed basal expression | ≥5.0 × 109 | |
| Phage Display | TG1 Phage Display Electrocompetent Cells | Antibody phage display with amber suppressor | ≥4.0 × 1010 |
| SS320 Phage Display Electrocompetent Cells | Antibody phage display | ≥5.0 × 1010 | |
| ER2738 Phage Display ElectroCompetent Cells | Peptide phage display with amber suppressor | ≥4.0 × 1010 | |
| Medium | IG® Recovery Medium | Superior formulation for all competent cell transformations | High-efficiency |
| IG® Autoinduction Medium | Promotes high-yield growth of E. coli and high-level expression of T7 promoter-regulated recombinant proteins | High-efficiency |
