Price range: $115.00 through $1,875.00
Achieve clean, efficient affinity-tag removal with IG® TEV Protease. This highly specific recombinant protease precisely cleaves proteins containing a TEV recognition sequence, such as ENLYFQG, while minimizing unwanted proteolysis. Its histidine tag enables easy removal by IMAC after digestion, helping streamline purification and preserve the structure and activity of your target protein.
Custom formats, package sizes and bulk pricing are available upon request.
IG® TEV Protease is a recombinant, highly specific protease used to remove affinity tags from fusion proteins. It recognizes the general sequence E-X-X-Y-X-Q-(S/G) and cleaves between the glutamine and serine or glycine residues. The most commonly used recognition sequence is ENLYFQG.
Derived from the tobacco etch virus (TEV) protease1,2, IG® TEV Protease contains a histidine affinity tag, allowing it to be easily removed from the cleaved target protein by immobilized metal affinity chromatography (IMAC). Its high sequence specificity makes it suitable for affinity-tag removal during recombinant protein purification while minimizing nonspecific cleavage.
Applications:
Benefits:
Product Source:
Recombinant protein derived from tobacco etch virus protease sequence.
Shipping Conditions:
Ship on dry ice or with ice packs.
Specifications:
Activity: 1 unit of TEV protease will digest 2 µg of a fusion protein with a TEV site in 1 hour at 25ºC. Completeness is evaluated by SDS-PAGE.
Components and Storage Temperature:
The following reagents are supplied with this product:
5022, 5025, 5027
| units | 2,000 units, 10,000 units, 100,000 units |
|---|
Quality Control:
1 unit of TEV protease will digest 2 µg of a fusion protein with a TEV site in 1 hour at 25ºC. Completeness is evaluated by SDS-PAGE.
General Guidelines:
Due to different protein properties, some constructs may require extended incubation periods with TEV protease (up to three days at either 4°C or 30°C) to achieve complete cleavage.
The addition of extra TEV Protease after 24 hours may also help achieve complete cleavage.
Extra TEV protease, up to 5 µL per 50 µL reaction volume, may help with some constructs.
Buffer Compatibility:
References:
TEV Protease Reaction Protocol:
NOTE: Protein constructs may affect the efficiency of TEV protease. Some cleavage reactions may take longer and protease enzyme quantities and reaction volumes may also be adjusted accordingly. For unknown samples, this simple procedure is a good place to start:
