RPA Optimization Enzymes & Kits

Comprehensive RPA Kits, Enzymes, and Lysis Solutions

Since 2016, Intact Genomics has pioneered the development and manufacturing of RPA-related kits and enzymes, enabling customers to build advanced diagnostic and molecular testing tools. Today, our RPA product portfolio includes comprehensive RPA kits, individual RPA enzymes and FastAmp® Viral and Cell Lysis Solutions. Through innovation, quality, and reliability, Intact Genomics empowers scientists and industry leaders to accelerate discovery and create next-generation molecular diagnostics.

OEM partnerships and bulk-order discounts are available. Please click here to read our white paper on RPA technology and Intact Genomics RPA products. If you have any questions or need purchasing or technical support, please feel free to contact us anytime at sales@intactgenomics.com.

Genomics kit with two vials
Product Links Package Size Features
IG® RPA Master Mix 25 rxns, 100 rxns, 500 rxns Delivers powerful isothermal amplification for molecular diagnostics and research. Easy-to-use, fast, reliable, and cost-effective.
IG® Recombinase Polymerase Amplification (RPA) Kit v2 25 rxns, 100 rxns, 500 rxns Provides faster and more discernible results for isothermal DNA amplification.
FastAmp® qRPA SYBR Kit 25 rxns, 100 rxns, 500 rxns Screens primer conditions to minimize non-template control (NTC) background.
FastAmp® Plant Direct RPA Kit 25 rxns, 100 rxns, 500 rxns Rapid DNA amplification from plant tissues at a constant temperature of 37°C.
IG® Recombinase Polymerase Amplification (RPA) Kit 25 rxns, 100 rxns, 500 rxns Contains the reagents and enzymes needed for DNA amplification.
T4 UvsX DNA Recombinase 100 μg, 500 μg, 1000 μg Important for error-free repair of DNA double-strand breaks and replication fork restart.
T4 UvsY Protein 100 μg, 500 μg, 1000 μg Phage T4 recombination mediator protein that stimulates filament nucleation.
T4 gp32 Protein 100 μg, 200 μg, 500 μg Single-stranded DNA-binding protein involved in DNA replication, recombination, and repair.
Bsu DNA Polymerase Large Fragment 1,000 units Polymerase useful for extending primers in RPA.
Sau DNA Polymerase Large Fragment 1,000 units Polymerase useful for extending primers in RPA.
Glycerol Free T4 UvsX DNA Recombinase 100 μg, 500 μg, 1000 μg Supports error-free DNA double-strand break repair and replication fork restart.
Glycerol Free T4 UvsY Protein 100 μg, 500 μg, 1000 μg Recombination mediator protein that stimulates filament nucleation.
Glycerol Free T4 gp32 Protein 500 μg Single-stranded DNA-binding protein for DNA replication, recombination, and repair.
Glycerol Free Bsu DNA Polymerase Large Fragment 1,000 units Polymerase useful for extending primers in RPA.
Exonuclease III 10,000 units, 25,000 units Digests one strand of duplex DNA from blunt ends, 5′ overhangs, or nicks.
Endonuclease IV (Nfo) 2,000 units, 5,000 units Functions as an apurinic/apyrimidinic nuclease and 3′-terminal di-esterase for DNA repair.
FastAmp® Viral and Cell Lysis Solution (1×, 5×) 25 reactions, 100 reactions Enables direct use of lysed DNA or RNA in RPA, RT-isothermal amplification, PCR, and RT-qPCR without extraction.
T4 phage enzyme DNA replication

Overview of the RPA Amplification Process

Recombinase Polymerase Amplification (RPA) amplifies DNA at a constant temperature (37-42 °C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX is used in combination with its accessory protein, UvsY.

The recombinase interacts with the primers to form nucleoprotein filament. This complex is able to bind with homologous double-stranded DNA through a strand exchange. After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu/Sau polymerase extends the primers, creating a new complete copy of the template, and amplification can continue like Polymerase Chain Reaction (PCR).

Applications and Benefits

  • Highly selective and sensitive isothermal amplification technique.
  • Alternative to PCR. No Thermocycler needed.
  • Speed and sensitivity. Excellent for Rapid Molecular and Agricultural tests. This isothermal reaction employing T4 UvsX DNA Recombinase does not require pretreatment of DNA, nor does it require any strand denaturation, making it suitable for field tests or performing tests with minimal equipment and supplies.
  • Successfully implementation with different detection strategies, from end-point lateral flow strips to real-time fluorescent detection.

Intact Genomics offers a variety of Recombinase Polymerase Amplification (RPA) optimization enzymes and kits. All enzymes thoroughly tested for activity, purity and concentration. We are continuously discovering and developing novel classes of RPA enzymes in order to speed up the point-of-care (POC) diagnostic process.