Tte UvrD Helicase

Tte UvrD is a thermostable, ATP-dependent DNA helicase derived from the thermophilic bacterium Thermoanaerobacter tengcongensis (Tte). The enzyme unwinds double-stranded DNA (dsDNA), generating single-stranded DNA (ssDNA) that can be accessed by other proteins, enzymes, primers, and probes.

Intact Genomics Tte UvrD is a key enzyme used in helicase-dependent amplification (HDA)¹. In HDA, Tte UvrD separates complementary DNA strands to generate single-stranded templates, allowing primer hybridization and DNA polymerase extension without the thermal cycling required for PCR. Studies have also shown that HDA may use the same primer sequences designed for PCR, helping simplify assay development².

Tte UvrD is a thermostable enzyme that originates from a thermophilic organism and it is active at temperatures up to 65 °C. It is suitable for HDA, DNA-unwinding studies, helicase activity assays, isothermal nucleic acid amplification, and other molecular biology applications requiring enzymatic strand separation.

Applications:

• Helicase-dependent amplification (HDA)
• Isothermal nucleic acid amplification
• DNA strand-unwinding studies
• Helicase activity assays
• Probe- and primer-based DNA detection workflows

 Benefits:

• Thermostable and active at temperatures up to 65 °C
• Generates single-stranded DNA without thermal cycling
• Supports primer hybridization and DNA polymerase extension
• May be used with PCR-designed primers, simplifying assay development
• Suitable for research and diagnostic assay development

 Source:

Recombinant UvrD expressed from a plasmid carrying the thermostable UvrD gene from Thermoanaerobacter tengcongensis.

 Specifications:

• Purified recombinant Tte UvrD helicase
• Molecular weight: 83 kDa
• Free of detectable endonuclease activity

Product Components and Storage Temperature:

 Custom formats and package sizes are available upon request.

Quality Control:

Nuclease Activity: DNA integrity is evaluated after overnight incubation with concentrated enzyme. The DNA must remain intact, with no detectable degradation, compared with a no-enzyme control.
Enzyme Activity: At 24 nM (2 ng/µL), the enzyme must pass both ATPase and fluorescence-based helicase activity assays and demonstrate performance comparable to an approved UvrD batch-control standard.

References:
1. An, L. et al. Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification *. Journal of Biological Chemistry 280, 28952–28958 (2005).
2. Gavrilov, M. et al. Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics. Nat Commun 13, 6312 (2022).