IG® RPA Master Mix – Lyophilized

IG® RPA Master Mix-Lyophilized is a ready-to-use, freeze-dried premix for rapid and sensitive isothermal DNA amplification. It contains T4 UvsX recombinase, UvsY recombination mediator, gp32 single-stranded DNA-binding protein, Bsu DNA polymerase, stabilizers, and an optimized reaction formulation.

After reconstitution, the master mix enables robust DNA amplification at temperatures as low as 25°C without thermal cycling. Results may be detected in as little as 5–10 minutes, with a typical reaction time of 10–20 minutes. Its proprietary, patent-pending formulation provides efficient target amplification with reduced background and off-target products, resulting in improved amplification specificity.

The lyophilized format simplifies reaction setup, storage, and transportation king it suitable for routine laboratory use and point-of-need testing. Users need only add primers, template DNA, and the required reaction components.

Figure 1: IG RPA Master Mix amplified DNA in 10 minutes at 25°C, in 5 minutes at 37°C.

Key Features:
•  Rapid isothermal DNA amplification at 25 °C
•  Results in as little as 5–10 minutes
•  Low background and reduced nonspecific amplification
•  Detection sensitivity below 1,000 copies per reaction
•  Convenient lyophilized format
•  No thermal cycler required
•  Suitable for laboratory and field-deployable assays

Applications:
•  Rapid pathogen detection
•  Molecular diagnostics research
•  Point-of-need nucleic acid testing
•  Food and environmental testing
•  Agricultural and veterinary testing
•  Genotyping and DNA screening
•  Development of lateral-flow and fluorescence-based assays

Specifications:
Enzymes: Purified and Nuclease free
Format: PCR tubes and 1.7 mL centrifuge vials
Shipping Condition: Shipped with frozen gel packs

Product Components and Storage Temperature:

*Store at –20 °C upon receipt. Keep the lyophilized product tightly sealed and protected from moisture. Product parts are stable at 4 ºC for at least for 1 month. Avoid room-temperature long-term storage simply because the product is lyophilized.

Quality Control:
All enzymes thoroughly tested for activity and purity.
All component proteins of the Enzyme Mixture are free from detectable nuclease activities.
RPA activity of each lot is validated to be consistent with prior IG® lots and to be equal to or better than major competitors in the market.
Product quality manage system is ISO 13485 certified.

General Guidelines:

• Setting up the reaction on ice is recommended for consistent performance.
• Mixing is very important to ensure a homogeneous reaction. Mix the reagents well by pipetting before using. Mix by pipetting half of the total volume 10 times.
• Low copy DNA targets may require longer incubation times, warming to 37 ºC, and/or higher template concentration.
• Primers are optimally 25-35 BP in length and do not form either homo– or hetero-dimers. Primers should be checked to minimize secondary structures, e.g., hairpins.