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i7® High-Fidelity DNA Polymerase 2x master mix is a ready to use premix which contains high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® High-Fidelity DNA Polymerase 2x master mix has the high-fidelity, sensitivity and processivity with an error rate ~2.8x102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes.