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Tte MutL Protein
Tte MutL Protein is a thermostable accessory protein from Thermoanaerobacter tengcongensis that interacts with Tte UvrD helicase and exhibits ATPase activity. It is suitable for studying DNA mismatch repair, MutL–UvrD interactions, helicase regulation, and for developing or optimizing helicase-dependent and other isothermal DNA amplification systems.
Custom formats, package sizes and bulk pricing are available upon request.
Price range: $495.00 through $1,425.00
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Tte MutL is a thermostable accessory protein associated with DNA helicases from Thermoanaerobacter tengcongensis (Tte). It is involved in prokaryotic DNA mismatch repair and is a known interaction partner of Tte UvrD helicase1.
Tte MutL possesses ATPase activity, and ATP hydrolysis serves as an indicator of enzyme activity. It may also enhance helicase-dependent amplification (HDA) under certain reaction conditions2. Its effects on helicase activity are typically evaluated in the presence of Tte UvrD helicase.
Intact Genomics’ Tte MutL is suitable for studies of DNA mismatch repair, MutL–UvrD interactions, ATPase activity, helicase regulation, and the development or optimization of helicase-dependent and other isothermal DNA amplification systems.
Applications:
- Development of isothermal DNA amplification systems including Helicase-dependent amplification (HDA)
- DNA helicase and strand-unwinding assays
- Studies of prokaryotic DNA mismatch repair
- Investigation of Tte MutL–Tte UvrD protein interactions
Benefits:
- Supports research and development of thermostable nucleic acid amplification technologies
- May improve HDA performance under optimized reaction conditions
- Useful for studying DNA repair, helicase activity, and protein–protein interactions
- ATPase activity provides a measurable indicator of enzyme function
- Suitable for research and diagnostic assay development
Source:
Recombinant MutL expressed from a plasmid carrying a thermostable Thermoanaerobacter tengcongensis MutL gene.
Specifications:
- Purified recombinant Tte MutL Protein
- Molecular weight: 67 kDa
- Free of detectable endonuclease activity
Product Components and Storage Temperature:
Custom formats and package sizes are available upon request.
Quality Control:
Nuclease Activity: DNA nuclease activity is evaluated by comparing the integrity of DNA incubated overnight with a concentrated enzyme sample to that of a no-enzyme control. No detectable DNA degradation should be observed.
Enzyme Activity: At a concentration of 400 nM, the enzyme passes an ATPase assay and demonstrates activity comparable to that of a standard Tte MutL batch.
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Protocol for DNA Helicase Activity:
NOTE: this procedure requires another IG® enzyme, Tte UvrD or Super-Tte UvrD
1. Set up the reaction according to the table.
2. Incubate the reaction mixture at 65 °C for 20 minutes. The incubation time and temperature may be adjusted for different applications. Typical conditions range from 42–68 °C for 10–60 minutes.
Note : ATP hydrolysis can limit helicase activity at higher temperatures.
3. To inactivate the enzyme, incubate the reaction at 80 °C for 15 minutes.
References:
Ban, C. & Yang, W. Crystal Structure and ATPase Activity of MutL: Implications for DNA Repair and Mutagenesis. Cell 95, 541–552 (1998).
An, L. et al. Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification*. Journal of Biological Chemistry 280, 28952–28958 (2005).
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