Sub-Total: $0.00
Glycerol-Free RNase Inhibitor, Murine
Intact Genomics (igĀ®) Glycerol-free RNase Inhibitor is a powerful tool to inhibit and control RNase contaminants in enzymatic manipulations of RNA. It is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C.
Price range: $170.00 through $381.00
Related products
-
IGĀ® sgRNA Synthesis Kit for Cas12a Nuclease
Price range: $246.00 through $1,183.00 Select options This product has multiple variants. The options may be chosen on the product page -
igScriptā¢ Reverse Transcriptase
Price range: $108.00 through $283.00 Select options This product has multiple variants. The options may be chosen on the product page -
IGĀ® Recombinase Polymerase Amplification (RPA) Kit V2
Price range: $243.00 through $2,125.00 Select options This product has multiple variants. The options may be chosen on the product page -
IGĀ® Recombinase Polymerase Amplification (RPA) Kit V1
Price range: $230.00 through $1,670.00 Select options This product has multiple variants. The options may be chosen on the product page -
IGĀ® XL1 Blue Max Electrocompetent E. coli Cells
Price range: $139.00 through $263.00 Select options This product has multiple variants. The options may be chosen on the product page
Product Description
Description:
RNase inhibitors are commonly used to inhibit and control RNase contaminants in enzymatic manipulations of RNA. Intact Genomics (igĀ®) Glycerol-free RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used withĀ TaqĀ DNA Polymerase, Reverse Transcriptase. Glycerol-free RNase Inhibitor from Murine is resistant to oxidation compared to the human/porcine RNase inhibitors as it lacks the pair of cysteine residues found in the human/porcine versions. In addition, it is stable at less than 1 mM DTT concentration.
Benefits of Glycerol-Free Enzyme:
- Optimal for Lyophilization:Ā Perfect for freeze-drying into diagnostic kits, ensuring stability and performance for point-of-care tests like molecular diagnostics.
- Better Compatibility:Ā Avoid interference in sensitive applications where glycerol limits solubility in certain organic solvents.
- Improved Performance: Enzyme formulations are easier to handle in high-throughput assays, as thereās no added viscosity affecting pipetting.
- Simplified Integration:Ā Allow for more flexible formulation, making it easier to add specific stabilizers or buffers for optimized performance in various systems.
You must be logged in to post a review.
Protein Source:
1. coli strain expressing recombinant RNase Inhibitor gene from mouse.
Applications:
- cDNA synthesis
- RT-PCR/RT-qPCR
- In vitro transcription/translation
- RNA labeling
- Microfluidics assays
Protein Purity:
The physical purity of this enzyme is ā„98% as assessed by SDS-PAGE with CoomassieĀ® blue staining.
Product Components & Storage:
- RNase Inhibitor, Murine, Glycerol-free : -80Ā°C
Enzyme Storage Buffer
- 50 mM HEPES, 750 mM KCl, 1 mM DTT, and 0.1 mM EDTA, pH 7.5
Heat Inactivation:
70ĀŗC for 20 minutes
Unit Definition:
One unit is defined as the amount of RNase Inhibitor is required to inhibit the activity of 5 ng of RNase A by 50%.
Quality Control:
SDS-PAGE purity, protein concentration, endodeoxyribonuclease assay, performance evaluation with RT-qPCR with activity meeting or exceeding reference enzyme.
"*" indicates required fields
Reviews
There are no reviews yet.