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Taq DNA Polymerase
Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.
Price range: $118.00 through $479.00
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Product Description
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).
E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Taq Polymerase Comparison Data
Applications
- Routing PCR cloning
- Primer extension
- Colony PCR
- Elongation efficiency 1.0-1.2 kb/min.
- Formulated for amplifying long target DNA.
- Efficient for amplifying high GC content DNA with Intact Genomics magic enhancer
Product Includes
- Taq DNA Polymerase
- 10x PCR Buffer with Mg2+
- 5x Magic Enhancer
- 10 mM dNTP (Cat. # 3241d, 3241d-04 only)
Storage Temperature
–20 °C
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 ºC
10x PCR Buffer with Mg2+
100 mM Tris-HCl pH 8.0, 15 mM MgCl2, 100 mM KCl, 80 mM (NH4)2SO4, 0.5% Igepal CA 630
Unit Definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 ºC.
References:
-
- Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. 127, 1550-1557.
- Lawyer, F.C. et al. (1993). PCR Methods and Appl. 2, 275-287.
- Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res. 18, 7317-7322.
- Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
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1. Thaw PCR buffer, dNTP, Primer solutions, 5x Magic Enhancer (if required) and mix thoroughly before use.
2. Prepare a reaction mix according to the following table: The reaction mix typically contains all the components needed for PCR except the template DNA.
3. Mix the reaction mixture thoroughly.
4. Add template DNA to the individual PCR tubes containing the reaction mixture.
5. Program the thermal cycler according to the manufacturer’s instructions.
A typical PCR cycling program is outlined in the following table.
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