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Taq DNA Polymerase 2x Premix
IG™ Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.
Price range: $144.00 through $273.00
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Product Description
IG™ Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, and stabilizers. It has been optimized for routine PCR applications.
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).
Product Source
E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Applications
- PCR
- Primer extension
- Colony PCR
Product Includes
- Taq DNA Polymerase 2x Premix with Dye
- 5x Magic Enhancer
Storage Temperature
–20 °C
1x Premix Composition
10 mM Tris-HCl pH 9.0 , 50 mM KCl, 1.5 mM MgCl2, 0.2 mM dNTP, 5% Glycerol, 0.08% Igepal CA 630, 0.05% TWEEN-20, 25 Units/ml Taq DNA Polymerase
Unit Definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 ºC.
Product Use Limitation and Disclaimers
This product is for research purposes only. It is not intended for therapeutic or diagnostic purposes in humans or
animals. This product contains chemicals which may be harmful if misused or direct human contact is made.
Intact Genomics is dedicated to practicing and maintaining science and technology ethics. Buyer agrees to use
the purchased materials in full compliance with applicable law and regulations.
Technical Support
Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.
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1. Thaw primer solutions, 5x Magic Enhancer (if required) and mix thoroughly before use.
2. Prepare a reaction mix according to the following table: The reaction mix typically contains all the components needed for PCR except the template DNA.
3. Mix the reaction mixture thoroughly.
4. Add template DNA to the individual PCR tubes containing the reaction mixture.
5. Program the thermal cycler according to the manufacturer’s instructions.
A typical PCR cycling program is outlined in the following table.
6. Place the PCR tubes in the thermal cycler and start the cycling program.
Reference
1. EChien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. 127, 1550-1557.
2. Lawyer, F.C. et al. (1993). PCR Methods and Appl. 2, 275-287.
3. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res. 18, 7317-7322.
4. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
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