Description

Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.

Applications

  • Strand displacement DNA synthesis (1)
  • Random primer labeling
  • Second strand cDNA synthesis
  • dA-tailing

Protein Purity
The physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).

Product Source
E. coli strain expressing Sau Polymerase I gene lacking the N-terminal exonuclease domain.

Product Includes
• Sau DNA Polymerase I, Large fragment
• 10x Sau DNA Polymerase I reaction buffer

1x Sau DNA Polymerase I reaction buffer
10 mM Tris-HCl
50 mM KCl
10 mM MgCl2
1 mM DTT
pH 7.9 @ 25°C

Storage Buffer
50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA,
50% Glycerol
pH 7.5 @ 25ºC

Storage Temperature
–20ºC

Heat inactivation
70°C for 20 min

Unit Definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 37º C.

Quality Control Assays
Sau Polymerase I, Large fragment is free from detectable nuclease activities.  Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.

References
1. Piepenburg, O. et al. (2006) PLOS Biology, 4, 1115–1121.3592, 3595