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Hot Start Taq 2x Master Mix
Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. Intact Genomics Hot Start Taq 2x Master Mix has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´-flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with the unique Intact Genomics 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.
$216.00
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Product Description
Applications
- Routine PCR and RT-PCR
- Primer extension
- Colony PCR
- Genotyping
- Efficient for amplifying high GC content template DNA with Magic Enhancer.
Product Includes
- Hot start Taq 2x master mix
- 5X Magic Enhancer
1x Master Mix Composition
10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl2, 0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Hot start Taq Polymerase.
References:
1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. 127, 1550-1557.
2. Lawyer, F.C. et al. (1993). PCR Methods and Appl. 2, 275-287.
3. Longley, M.J., Bennett, S.E and Mosbaugh D.W. Nucleic Acids Res.18, 7317-7322.
4. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
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Protocol
1. Prepare a reaction mix according to the following table:
2. Mix the reaction mixture thoroughly.
3. Program the thermal cycler according to the manufacturer’s instructions.
4. A typical PCR cycling program is outlined in the following table.
5. Place the PCR tubes in the thermal cycler and start the cycling program.
6. Analyze 5 μl of PCR products by agarose gel electrophoresis.
References:
1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. 127, 1550-1557.
2. Lawyer, F.C. et al. (1993). PCR Methods and Appl. 2, 275-287.
3. Longley, M.J., Bennett, S.E and Mosbaugh D.W. (1990) Nucleic Acids Res.18, 7317-7322.
4. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
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