ig® HMW DNA Extraction Kit is designed for isolation of high-molecular-weight (HMW) DNA from white blood cells, plant tissue and Gram-positive and Gram-negative bacteria. DNA purified with the HMW DNA Extraction Kit is particularly suitable for long-read sequencing platforms.

Key Benefits

  • Optimized and quick protocol with no hazardous/toxic chemicals
  • Simplified preparation steps for extracting high-purity HMW DNA upto 1 megabase
  • Best for long-read sequencing

HMW DNA Extraction Kit

Figure 1.   Examples of high molecular weight (HMW) genomic DNAs prepared with ig® HMW gDNA preparation kit. In example samples 1 & 2, the majority of HMW gDNA is >30 ~ 300 kb, and some are up to megabase in size. M. Lambda ladder DNA marker.

Kit Components and Storage

The below reagents should be stored at –20°C.

  • Proteinase K Solution (20mg/ml)
  • RNase A solution (100 ng/ml)
  • Lysozyme Solution (50 mg/ml)

The below reagents should be stored at 4°C.

  • PBS
  • HMW Blood Lysis Buffer
  • HMW Lysis Buffer A
  • Protein Precipitation Solution

Not included but required:

  • TE
  • Technical SupportIntact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.