ig® HMW DNA Extraction Kit is designed for isolation of high-molecular-weight (HMW) DNA from white blood cells, plant tissue and Gram-positive and Gram-negative bacteria. DNA purified with this system is particularly suitable for long-read sequencing platforms.
- Optimized and quick protocol with no hazardous/toxic chemicals
- Simplified preparation steps for extracting high-purity HMW DNA upto 1 megabase
- Best for long-read sequencing
Figure 1. Examples of high molecular weight (HMW) genomic DNAs prepared with ig® HMW gDNA preparation kit. In example samples 1 & 2, the majority of HMW gDNA is >30 ~ 300 kb, some are up to megabase in size. M. Lambda ladder DNA marker.
Kit Components and Storage
The below reagents should be stored at –20°C.
- Proteinase K Solution (20mg/ml)
- RNase A solution (100 ng/ml)
- Lysozyme Solution (50 mg/ml)
The below reagents should be stored at 4°C.
- HMW Blood Lysis Buffer
- HMW Lysis Buffer A
- Protein Precipitation Solution
Not included but required: