Description
IG™ Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.
IG™ Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, and stabilizers. It has been optimized for routine PCR applications.
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).
Product Source
E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Applications
- PCR
- Primer extension
- Colony PCR
Product Includes
- Taq DNA Polymerase 2x Premix with Dye
- 5x Magic Enhancer
Storage Temperature
–20 °C
1x Premix Composition
10 mM Tris-HCl pH 9.0 , 50 mM KCl, 1.5 mM MgCl2, 0.2 mM dNTP, 5% Glycerol, 0.08% Igepal CA 630, 0.05% TWEEN-20, 25 Units/ml Taq DNA Polymerase
Unit Definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 ºC.
Technical Support
Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.
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