High Fidelity DNA Polymerases
Intact Genomics (IG) i7 High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8×10^2-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. We have tested i7 High-Fidelity DNA Polymerase activity with λ DNA and other difficult templates for PCR amplification up to 20kb. Intact Genomics (IG) variety of i7 high-fidelity DNA Polymerase products generate robust and high-quality PCR products.
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i7® High-Fidelity DNA Polymerase has the high-fidelity, sensitivity and processivity with an error rate ~2.8x102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes. -
i7® High-Fidelity DNA Polymerase 2x master mix is a ready to use premix which contains high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® High-Fidelity DNA Polymerase 2x master mix has the high-fidelity, sensitivity and processivity with an error rate ~2.8x102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes.
